Structure, function and growth of prokaryote and eukaryote cells (ii) Cell growth and Cell cycle Interphase Mitosis Mitotic index Control of the cell cycle Abnormal Cell division: cancer cells
Cell Cycle When cells reach a certain size they must stop growing or divide. The cell cycle is the period from the formation of a new cell until that cell divides itself The length of the cycle depends on the cell – but is usually 8 – 20 hours
The Cell Cycle CK
Interphase… G 1 (First Gap Period) Cell grows and increases in mass Cell membrane (+ wall) are increased Number of organelles increase Chemical preparation for DNA Synthesis (enzymes, deoxyribose sugars, nucleotide bases and phosphates synthesised)
Interphase S phase (synthesis) Replication of DNA Chromosomes held together by centromere In animals centriole pairs duplicate
Interphase G 2 (Second gap phase) Cell builds up energy reserves Increased protein synthesis
Mitosis Revision – complete the mitosis diagram (note this is from standard grade)
Mitosis Compare this to your SG diagram. Name the stages Interphase Prophase Metaphase Telophase Cytokinesis What features characterise each stage?
Mitosis Prophase Chromosomes condense (coil and fold up) to become visible. Joins onto spindle fibre Chromatids in condensed form
Mitosis – prophase cont… Cytoskeleton disintegrates (its components (microtubules) are used to begin spindle formation) Spindle formation begins between centrosomes (aka Microtubule organising centres – MTOCs) Nuclear Membrane starts to break up
Mitosis – prophase cont…
Mitosis Metaphase Spindle continues to grow from centrosomes (which are now at the poles) Chromosomes line up across the centre of the cell
Mitosis – metaphase cont.. Chromosomes attach to the spindle fibre by the kinetochore in the centromere The arrangement of the chromosomes across the equator is also known as the metaphase plate.
Mitosis – metaphase cont…
Mitosis Anaphase Centromeres divide Sister chromatids separate and the chromosomes are pulled to the poles
Mitosis – anaphase cont…
Mitosis Telophase Chromosomes begin to de-condense Nuclear membrane reforms Spindle disassembles and reassembles as the cytoskeleton in the 2 new cells
Mitosis – telophase cont…
Cytokinesis Dividing of the cytoplasm and organelles Animals Cytoplasm is pinched by a ring of contractile microfilaments (made up of the proteins actin and myosin) As the ring gets smaller it forms a cleavage furrow
Cytokinesis cont…
In plants Membrane vesicles spread across the equator of the cell. They merge to form plasma membrane The new membranes lay down the cell wall between the two cells.
Cytokinesis cont…
Mitotic Index The fraction (or percentage) in a microscope field that contain condensed chromosomes (i.e. Undergoing mitosis)
Learning Activities Look at web animations Cell biology – Mitosis / Meiosis – How cells divide Posters Card sort (pictures / names / descriptions) Read Dart pg 9-14 and take notes Use Scholar Advanced Higher Questions