Unique Microplates for UVStar® Unique Microplates for the UV-Range

Pre-UVStar®-Period At my time, only quartz glass was available, and it was fragile expensive Microplates hard to clean for reuse limited in throughput

Uniqueness of UVStar® Microplates with outstanding optical properties Dedicated for OD reading in the UV-range Proprietary, unique resin Optical window down to 200 nm Available in 96 and 384 well format

Prerequisites for UVStar® Availability of a unique proprietary plastic Material free of any UV-blocking additives Production of high quality films (140 µm) Lamination process free of glue and solvents Adaptation of µClear®-process to UVStar® UVStar_Rev. 1

µClear®-Technology Direct lamination technology Process free of glue or solvents 50 - 500 µm films Enables multi-mode detection Worldwide Patents Germany: DE 19712484 Europe: Pat. pending USA: Pat. pending

Applications of UVStar®-Plates Optical window down to 200 nm Background: < 60 mE @ 260 nm DNA-quantification @ 260 nm Purity of DNA @ 260/280 nm Protein concentration @ 280 nm

Absorbance of DNA vs. Protein

Clear Bottom Plates 1 mm bottom 140 µm bottom bonding of glass sheets bonding of plastic films 2 component injection molding

Advantages of µClear®-Technology

From µClear® to UVStar®

Absorbance of UV-transparent Resins Greiner vs. Competitors

UV-Light Absorbance 384 well µClear® vs. UVStar®

Measurement of DNA-Conc. 96 vs. 384 Well UVStar®-Plates

Cell Growth (WST) in TC-treated 384 Well UVStar®-Plates

Growth of CHO-Cells in TC-treated 96 Well Plates

Homogeneity of 384 Well Plates

Performance of Background 384 Well UVStar® @ 260 nm

Drift and Background Greiner vs. Competitor

Drift and Background Greiner vs. Competitor Mean 0,0524 Standard Deviation 0,002 Correlation of Variation 4 % Mean 0,0498 Standard Deviation 0,001 Correlation of Variation 2 %

Pathlength & Lambert Beer’s Law I = Io ·e-k’ · c · x Well pathlength [x]: not defined Cuvette pathlength [x]: fixed

Correlation Pathlength vs. Absorption @ 260 nm Quartz Cuvette [10 mm] = 0,08 OD UVStar_Rev. 1

Chemical and Thermal Resistance UVStar_Rev. 1

Benefits of UVStar®-Plates Available in a 96 well and 384 well format Extended optical window from 200 to 1000 nm Background less than 0.060 E @ 260 nm Minimized variation from well to well Ideal for quantifying DNA and total protein No more need for expensive/fragile quartz plates

UVStar® Made by