Restriction mapping revision

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Restriction Analysis of Plasmids

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Presentation transcript:

Restriction mapping revision

16. Construct a restriction map of a linear fragment of DNA, using the following data. Your map should indicate the relative positions of the restriction sites along with distances from the ends of the molecule to the restriction sites and between restriction sites: DNA Sizes of Fragments (bp) uncut DNA 10,000 DNA cut with EcoRI 8000, 2000 DNA cut with BamHI 5000, 5000 DNA cut with EcoRI + BamHI 5000, 3000, 2000

DNA Sizes of Fragments (bp) uncut DNA 10,000 DNA cut with EcoRI 8000, 2000 DNA cut with BamHI 5000, 5000 DNA cut with EcoRI + BamHI 5000, 3000, 2000

17. Construct a restriction map of a linear fragment of DNA, using the following data. Your map should indicate the relative positions of the restriction sites along with distances from the ends of the molecule to the restriction sites and between restriction sites: DNA Sizes of Fragments (bp) uncut DNA 900 DNA cut with EcoRI 700, 200 DNA cut with HindIII 600, 300 DNA cut with BamHI 500, 350, 50 DNA cut with EcoRI + HindIII 600, 200, 100 DNA cut with EcoRI + BamHI 500, 200, 150, 50 DNA cut with HindIII + BamHI 500, 250, 100, 50

DNA Sizes of Fragments (bp) uncut DNA 900 DNA cut with EcoRI 700, 200 DNA cut with HindIII 600, 300 DNA cut with BamHI 500, 350, 50 DNA cut with EcoRI + HindIII 600, 200, 100 DNA cut with EcoRI + BamHI 500, 200, 150, 50 DNA cut with HindIII + BamHI 500, 250, 100, 50

18. Construct a restriction map of a circular DNA plasmid, using the following data. Your map should indicate the relative positions of the restriction sites along with distances between restriction sites: DNA Sizes of Fragments (bp) uncut DNA 7950 DNA cut with BglII DNA cut with EcoRI DNA cut with HpaI DNA cut with BglII + EcoRI 5416, 2534 DNA cut with BglII + HpaI 6632, 1318 DNA cut with EcoRI + HpaI 4098, 3852

Sizes of Fragments (bp) 18. Construct a restriction map of a circular DNA plasmid, using the following data. Your map should indicate the relative positions of the restriction sites along with distances between restriction sites: Draw your answer on paper and compare it to the answer you receive when you click the Check Your Answers button. DNA Sizes of Fragments (bp) uncut DNA 7950 DNA cut with BglII DNA cut with EcoRI DNA cut with HpaI DNA cut with BglII + EcoRI 5416, 2534 DNA cut with BglII + HpaI 6632, 1318 DNA cut with EcoRI + HpaI 4098, 3852