1. Restriction Map: 1 PstI PstI + CcoIV 700030007000 4000 PstI CcoI 7Kbp 1 cut 2 cuts 3Kbp 4Kbp

2. Restriction Map: 2 EcoRi EcoRI + BstEII 200100 800200900 700 1,000 1 cut 2 cuts EcoRI 200 800 BstEII 100 900 100 900 100900

3. Restriction Map: 3 XhoI XhoI + FstII 1,000 3,000 5,0002,00014,000 11,0003,000 11,000 FstII 17,000 14,0003,000 2 cut 3cut 1 cut XhoI 5,0001,00011,000 2,0003,000or

4. GO TIME!

5. Restriction Map 1 Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. EcoRI EcoRI + HindIII 7,0006,00010,000 3,0003,000 1,000 HindIII EcoRI 10K 1K 6K EcoRI = 2 sites HindIII = 1 site EcoRI 3k 10k 10k 10k 10k 7k 1.) Linear or Plasmid ? 2.) What’s the Size? 3.) How many cuts? 4.) Easy Cuts First! 5.) Find relationships! 6.) Rotate =10K = 3 sites =HindIII Linear: Fragments = cuts + 1 Plasmid : Fragments = cuts

6. Restriction Map 2 Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. HhaIIHhaII +XhoI 6,0004,0006,000 2,000 HhaII XhoI 6K 2K 4K HhaII = 1 site XhoI = 1 site

7. Restriction Map 3 A RIVER runs from Stanton to Fredericksburg. Two Cabins are located on this stretch of river. The Teelin’s cabin and the Larson’s cabin are 5 km apart. The Larson’s cabin is 8 km from Stanton while the Teelin’s cabin is 11 km from Fredericksburg. make a map Using the data, make a map of the river and the cabins located along it. Use a horizontal free line to represent the river and symbols to represent the cabins. Label the symbols and intervals between symbols with the measurements of distance between the cabins. –How far is the Teelin’s cabin to Stanton? –How far is the Larson’s cabin to Fredericksburg? –Is there an alternate Map?

8. Stanton Larson’s Teelin’s Fredericksburg 8 km 5 km 11 km Stanton Larson’s Teelin’s Fredericksburg 8 km 11 km 5 km How far is the Teelin’s cabin to Stanton? How far is the Larson’s cabin to Fredericksburg? Is there an alternate Map? 13Km 16Km You Betcha, See Below! Now how far is the Teelin’s cabin to Stanton? Now how far is the Larson’s cabin to Fredericksburg? 3Km 6Km

9. Restriction Map 4 make a map There are towns along highway 28 running east to west. Using the data below, make a map of highway 28 and the towns located along it. Use a free horizontal line to represent highway 28 and symbols to represent towns. Label the intervals between the towns with the measurements of distance. Freeville : Mt. Crumpit 160 kmFreeville : Mt. Crumpit 160 km Mt. Crumpit : Whoville200 kmMt. Crumpit : Whoville200 km Waterville : Freeville120 kmWaterville : Freeville120 km Whoville : Waterville240 kmWhoville : Waterville240 km Mt. Crumpit : Waterville 40 kmMt. Crumpit : Waterville 40 km Freeville hunters get to start each hunting day 8 min. sooner than Waterville hunters.Freeville hunters get to start each hunting day 8 min. sooner than Waterville hunters.

10. East West 120K 40K 200K Freeville Waterville Mt. Crumpit Whoville

11. Restriction Map 5 Construct a Linear restriction map of the following digest. Include cuts and fragment sizes. SamIIISamIII +TrpII 13,00010,00017,000 9,0009,00010,000 5,0005,000 3,000 TrpII SamIII 10K 10K 17K 3K 5K 9K 27K 27K 27K SamIII = 2 sites TrpII = 1 site 13K

12. Restriction Map 6 Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. CabII CabII + MtGI 6,0004,5008,000 3,0002,5002,000 1,0001,500 1,000 10K 500 8K CabII = 3 sites MtGI = 2 site 500 MtGI 2K 10K 10K 10K CabII 1,500 CabII 2,500 1,000 4,500

13. Restriction Map 7 3.There is a trail that runs along a mountain ridge in eastern Vermont from James Camp to Camp Five. Using a horizontal line to represent the trail and dots to represent climbing features. Label the intervals between climbs with the measured distance. Blood Lake : Washington Peak3 km Washington Peak : Misery Rock4.5 km Hangman's Cliff : One Way Jacks Trail 1 km James Camp : Jackson's Thrill2 km Hangman’s Cliff : Misery Rock9.5 km Washington’s Peak : Jackson’s Thrill4 km Jackson’s Thrill : Misery Rock.5 km One Way Jacks Trail : Jackson’s Thrill10 km Blood Lake : Camp Five6.5 km Jackson’s Thrill should be climbed in the sun before the sun sets behind Washington’s Peak

14. James Camp Misery Rock Jackson’s Thrill Washington’s Peak Blood Lake Hangman’s Cliff One Way Jack’s Trail Camp V 1K3.5K2K3K4K.5 K 1.5K

15. Cow Person Superhero samurai Pirate

16. Problem set 1: # 1 EcoRI EcoRI + BamHIBamHI 10,0008,00010,000 2,000 10K EcoRI BamHI 2K 8K

17. Problem set 1: # 2 EcoRI EcoRI + BamHIBamHI 8,0006,00012,000 4,000 2,000 12K EcoRI BamHI 2K 4K EcoRI 6K

18. Problem set 1: # 3 EcoRI EcoRI + BamHIBamHI 8,0006,500 4,000 5,500 1,000 500 12K EcoRI BamHI 1K 6.5K EcoRI 4K 500 BamHI

19. Problem set 1: # 4 ClaI ClaI HindIII ClaI + HindIIIHindIII 11,0008,00014,000 8,0007,000 2,0004,000 2,000 ClaI HindIII ClaI HindIII 8000 2000 4000 7000 = 1site ClaI =2 sites

20. Problem set 1: # 5 HindIII HindIII BamHI HindIII + BamHIBamHI 15,000 18,000 10,0007,00013,000 6,000 3,000 HindIII HindIII = 2 sites BamHI =1 site 15000 300070006000 BamHI HindIII

21. Problem set 1: # 6 Pvul Smal Pvul Smal + PvulSmal 8,8008,00011,800 8,0005,5008,700 6,7004,5007300 60003,8004200 550032003000 2800 2200 2000 SmaI SmaI = 4 sites PvuI PvuI 3200 55002800 PvuI SmaI 45002000 PvuI 22003000 SmaI SmaI 3800 PvuI 8000

22. Problem set 2: # 7 EcoRI EcoRI BamHI EcoRI + BamHIBamHI 6,0004,5008,000 3,0002,5002,000 1,0001,500 500 BamHI 1,000 = 2site EcoRI =3 sites BamHI BamHI EcoRI EcoRI EcoRI 2,500 500 1,500 4,500

23. Problem set 2: # 8 EcoRI EcoRI + BamHI BamHI BamHI+ HindIII HindIII HindIII + EcoRI 18,0009,00011,5009,00016,30014,500 5,0008,0009,0008,700 5,000 4,0005,0008,0004,5003,500 1,5004,0003,5002,200 1,5002,8001,800 1,0001,500 EcoRIEcoRI HindIII BamHI3,5004,5009,000 BamHI 1,000 HindIII 1,8002,2005,0001,500 EcoRI 8,000 4,000

24. Problem set 2: # 9 EcoRI EcoRI BamHI EcoRI + BamHIBamHI 9,0008,00011,000 6,000 7,000 3,000 1,000 EcoRI BamHI EcoRI BamHI 6,000 1,000 8,000 3,000 = 1site EcoRI =2 sites

25. Problem set 2: # 10 EcoRIEcoRI + BamHI BamHIHindIIIHindIII + EcoRI ClaI + EcoRI ClaI 14,0009,00017,00012,00011,00014,00019,000 9,0008,0006,00011,0009,0005,0004,000 6,0003,0004,000 BamHI HindIII EcoRI ClaI 6,0005,0003,0005,0004,000

26. Quick Draw Δ (linear) SnaTII SnaTII BkaI SnaTII+ BkaIBkaI 16,00015,00018,000 15,00013,000 3,000 SnaTII 15,000 3,00013,000 BkaI 31,000

27. Quick Draw Σ (Plasmid) TtHII TtHII ZtoPI TtHII + ZtoPIZtoPI 8,5008,0008,500 500 TtHIIZtoPI8,000 500 8,500

28. Quick Draw Ω (linear) SpaZIII SpaZIII HmmI SpaZIII + HmmIHmmI 115100130 403015 10 SpaZIII 1030 100 HmmI HmmI 15 155

29. Quick Draw β (Plasmid) RamII RamII PhaTI RamII + PhaTIPhaTI 8,500 11,000 2,5002,000 500 RamII PhaTI 2,000 500 8,500 RamII 11,000