1. Genetics practical exam final Name Number

2. Section 1: Q1: In karyotpes A and B mention the abnormalities seen and the name of the disease in karyotype A and if known the name of the disease in B Q2:Describe the kind of disorders that can be diagnosed by cytogentic means Q3: Why are cells cultured before cytogentic analysis? Q4: Why do we add a hypotonic solution to cultured cells prior to cytogentic analysis? Q5: Why are cells dropped from a distance on glass slides in cytogentics? X X Y A) B)

3. Section 2: Q1: Design an appropriate PCR program to amplify a genomic fragment 1205bp long using the following primers Primer 1 5'-GCTACGGAAATCATGACTAC-3' Tm 58 Primer 2 5'-CGCTATCTACGGCATTATCC-3' Tm 53 Q2: In your own words, what could result form the establishment of strong dimer structures between a primer pair? Q3: Why do we add taq polymerase to the PCR? Q4: What could result if we accidentally did not add one of the primers to the PCR? Q5: In the following sequence design DNA primers to amplify the area in bold AAGAATCATTCTGTCACCTGACCCCAATTCCTTGACCTTGTTACAGAAAAGGTTTATGTA ATTATTGCAAGACATCTACATATCCCTACATAGATGGAAAACAATAATACATCTTATACA TACATATACTATTCATTTTTCACATGTAAGTAGGTGTTGTAATCAATATGACACAAATGG TTTTAATAACATTAAATTTATTTGACAGTATGTATTTTCTTATTCAATATGTAAATATAG GCAAGAGATTTTACTACTATCATGAGACATTTATGAATTCTTAGAAAGGAATTCTCACTT TTTAAAAACAATTGGGAATTGCTAATGTAAATAATGATGGTTCCTTGTGGTCAGCAAAAT GGAAAGAAACATGGAAGGGGTCGGAAATATGAAGGCACTTACACACACACACACACACGC ACACAAAAGCTATCAATTTCTGTCAGAAAATTGGCTGACGCATCCTTGCTTTACCTTGGA GCTATGGGAGGTAGCTCTTGATTTAGCTGCTTAATTTAGCATGGGTGTTAAAGAACCCTG TGTCAGCAAACTCACAGGAAGAACTTGTCATTTCTATTGGTATTCTGTCTAAGCTTCAAA

4. 5kb 10.5kb 11kb 3kb 1kb Section 3: Q1: The following plasmids (A, B and C) have bean digested using EcoRI and BamHI (the restriction sites are indicated on the plasmids) using your pin draw the predicted bands in gel picture below with consideration to size (A) (B) (C) 1kb BamHI EcoRI 4kb 6kb 0.5kb BamHI HindIII EcoRI 2.5kb 6kb 12kb 10kb 9kb 8kb 6kb 4kb 3kb 2kb 1kb 0.5kb 0.4kb 0.3kb (A) (B) (C)

5. Q2: Match restriction ends from column A to there corresponding end in column B A) CTGATGCC GACTA CTCGCT GAGCGA GGTAC CCATGTAA GCTGGCG CGAC B) ATTGGCGC CCGCG CATTA CGCGTAAT CGTC CGGGCAG ATATAG TATATC Section 4: Q1:What is the figure above called? Q2: How many genes are present in the figure? Q3: How may interons and exons are in each gene?