Effect to temperature on urease Lab report Effect to temperature on urease Member : Pim Pat Gail First Max mandarin 10-2

In this experiment help us to investigate the enzyme urease by using the temperature to observed the substrate concentrations affect enzyme activity.

Procedure 1 -2 drops of the critic acid with indicator change their color to light pink. -2 drops of baking soda with the indicator made the color change from purple to blue. -2 drops of dH20 made the indicator change their color to become lighter.

1. Why is it important to have the three reference tubes. 2 1. Why is it important to have the three reference tubes? 2.Can you predict what color of the solution will be if the urease enzyme convert urea to ammonia?

Test tube Color change 2 drops of critic acid with an indicator 2 drops of baking soda with an indicator Color change Purple to light Pink Purple to Blue Color get lighter

Procedure2 >>Urea + Indicator, the color stays the same and not change. >>Enzyme + Indicator, the color didn't change >>Urea + Enzyme + Indicator, change from purple to blue.

1. What are the purpose of procedure 2. 2 1.What are the purpose of procedure 2? 2.Why do we need to add an enzyme into a test tube without urea even though we can predict that o enzyme activity will occur?

Urea + Enzyme + Indicator Test Tube Urea + Indicator Enzyme + Indicator Urea + Enzyme + Indicator Color change No change Purple to Blue

Procedure 3 >>2 ml of Soya suspension before adding Urea and Indicator Test tube in ice bath: Remain normal Test tube at room temperature(25°C): Remain normal Test tube in water bath (70°C): enzyme denatured Test tube at 50°C temperature (45°C): Remain normal Test tube in boiling water: Enzyme denatured

Enzyme denatured (Remain the same) Test Tube Ice bath Room temperature Water bath 50 temperature Boiling water Temperature°C 25 70 45 100 Color change Remain normal enzyme denatured Enzyme denatured (Remain the same)

1.How does the temperature affect the urease activity? How do you know ? 2.From your result, what temperature would you use and avoid if you have to conduct an experiment using urease enzyme and why? 3.Descrive the appearance of the denatured urease enzyme in the test tube?

Result after adding Urea and Indicator When return into the test tube: Test tube in ice bath: Stay the same (purple) Test tube at room temperature(25°C): Does not denatured and change from purple to green Test tube in water bath (70°C): Not all denatured (We can see that active site can process). Test tube at 50°C temperature (45°C): Enzyme not denatured, change from purple to green Test tube in boiling water: Enzyme denatured, stay the same

Observed the color change after 5 minutes Test tube in ice bath: Turned from purple to light blue. We figured out that enzyme working when the temperature get warmer. Test tube at room temperature(25°C): Does not denatured and change from green to dark green Test tube in water bath (70°C ): Not all denatured, turned to green (We can see that active site can process). Test tube at 50°C temperature (45°C): Enzyme not denatured, change from green to dark green Test tube in boiling water: Enzyme denatured, stay purple.

Conclusion -We have learn how much temperature will kill enzyme by putting 4 test tube in 4 different temperatures. -It have some like when put enzyme at room temperature the bigger was open stove first then it make the temperature go very high we need to be careful before doing experiment -The prediction that we make before was enzyme will be survive in every temperature but it doesn't when we make experiment it can't be survive at 100 degree Celsius and at 0 degree Celsius it was died but not all of them.