1. 4.1 (c) To conduct a qualitative test for reducing sugar

2. Before you start get or make a copy of this Table of results
Sample
Initial colour
Final colour
A – Glucose solution
B – Water

3. Label two boiling tubes A and B.
Step 1
Label two boiling tubes A and B.

4. Place 2 ml of glucose solution into tube A.
Step 2
Place 2 ml of glucose solution into tube A.

5. Place 2 ml water into tube B.
Step 3
Place 2 ml water into tube B.

6. Add 2 ml of Benedict’s solution into each tube and swirl.
Step 4
Add 2 ml of Benedict’s solution into each tube and swirl.

7. Place both tubes in a water bath and heat for 5 minutes.
Step 5
Place both tubes in a water bath and heat for 5 minutes.

8. Expected Result
Positive: brick red
Negative: blue

9. Step 6 Table of results Sample Initial colour Final colour
A – Glucose solution
B – Water

10. END