1. EXPERIMENT: Action of Amylase on Starch

2. A B C D E F G Add 10 ml of distilled water to each tube.

3. A B C D E F G Add 7 drops of iodine solution to each tube.

4. A B C D E F G Add 1 ml of 1% soluble starch solution to tube A. ***Starch + iodine =

5. Place 10 ml 1% soluble starch solution in a 50 ml beaker and add 2 ml of 2% amylase solution, stirring as you add. Start timing after you finish adding the amylase. starch amylase

6. A B C D E F G After 2 minutes, add 1 ml of the starch-amylase solution to tube B.

7. A B C D E F G After 2 more minutes (4 minutes from time zero), add 1 ml of starch-amylase solution to tube C.

8. A B C D E F G Repeat this process every 2 minutes for the rest of the tubes.

9. A B C D E F G Observe the colors and record your observations.

10. Add remaining starch-amylase solution into test tube 1. 1

11. Add an equal amount of original 1% soluble starch solution into test tube 2. 1 2

12. Add 2 ml of Benedict’s solution to each tube. 1 2

13. Heat in water bath for 3 to 5 minutes. 1 2

14. Observe color change. Orange = sugar. 1 2