1. CATALASE LAB
ENZYME REACTION LAB

2. NORMAL CATALASE ACTIVITY
Reaction rate of 4
Room temperature
What did the bubbling represent?

3. REUSABILITY OF CATALASE
Same piece of liver was placed in new hydrogen peroxide
Did the catalase cause bubbling and speed up the reaction?

4. OCCURRENCE OF CATALASE
Apple, chicken, and potato were added to hydrogen peroxide
Compare the reaction rates (bubbling)
Is catalase found in other living tissues?

5. EFFECT OF TEMPERATURE ON REACTION RATE
Catalase and hydrogen peroxide were placed in temperatures of 0, 37, and 100 degrees Celsius (room temperature was previously done)
Reaction rates were compared
Does temperature have an affect on reaction rate of catalase with hydrogen peroxide?

6. EFFECT OF pH ON REACTION RATE
Catalase was added to neutral, acidic, and alkaline (basic) solutions of hydrogen peroxide
Compare the reaction rates
Does pH have an affect on reaction rate of catalase with hydrogen peroxide?

7. EFFECT OF AN INHIBITOR
An inhibitor is a molecule that can fit onto the active site of the enzyme, but can’t react (this is competition for the substrate)
Hydroxylamine (the inhibitor) was added to the catalase and hydrogen peroxide
Compare reaction rates of with and without the use of an inhibitor
Does an inhibitor affect reaction rate of catalse with hydrogen peroxide?

8. LAB WRITE-UP Title OMIT for this lab only
Problem (each part of the lab needs one)
Hypothesis (each part of the lab needs one)
Procedure (each part of the lab needs a short and concise paragraph
Continue with next slide

9. OBSERVATIONS
2 graphs
4 charts

10. CONCLUSIONS
Introduction paragraph discussing the reaction (what are enzymes and their functions in general, what is the enzyme, substrate, and products in this lab, what are you measuring?) ELABORATE!
Discuss what a normal reaction is (What does the bubbling represent?)
Discuss whether catalase is reusable or not, and how do you know (support with data)

11. CONCLUSIONS
Discuss whether catalase occurs in any other tissue or not (support with data)
Discuss effect of temperature on reaction rate (include optimal temperature), support with data
Discuss effect of pH on reaction rate (include optimal pH), support with data

12. CONCLUSIONS
Discuss effect of inhibitor on reaction rate, support with data
Discuss any errors and what each has caused
Summarizing paragraph concluding enzyme reactions and effects on their reactions