a b Supplementary Figure S1. Oh et al. 198 115 90.5 1 2 3 IP: HA WCE + Hdm2 HA-Ub FLAG-hTERT MG132 - WB: FLAG WB: Hdm2 hTERT -(Ub)n MG132 Hdm2 HA-Ub FLAG-hTERT - + - - + - - + - + + - + + + + + + + + 198 hTERT -(Ub)n IP: FLAG 115 90.5 WB: HA WB: FLAG WB: FLAG WCE WB: Hdm2 1 2 3 4 5 6

a b d c Supplementary Figure S2. Oh et al.  Ring 198 115 90.5 1 2 3 4 IP: FLAG WCE hTERT -(Ub)n WT  Ring + Hdm2 HA-Ub FLAG-hTERT MG132 - WB: HA WB: Hdm2 WB: FLAG 115 198 90.5 1 2 3 4 IP: FLAG WCE hTERT -(Ub)n WB: HA WB: Hdm2 WB: FLAG WT C464A + Hdm2 HA-Ub FLAG-hTERT MG132 - WB: Hdm2 WB: HA WCE IP: HA 1 2 3 4 Hdm2 C464A hTERT-HA + - c - wt C464A + 1 2 3 WB: Hdm2 WB: HA WB: GFP Hdm2 C464A hTERT-HA

a b Supplementary Figure S3. Oh et al. 294-491 WT 1-301 Myc-Hdm2s His-Ub hTERT-HA MG132 - - - + + + + + + 1-301 294-489 NC PC Myc-Hdm2 wt hTERT - + + + + PD: Ni-NTA 198 hTERT -(Ub)n 115 WB: HA WB: HA 50 bp WT 115 WCE 1-301 IC (36bp) 90.5 294-491 61.5 WB: Myc 1 2 3 4 5

a b Supplementary Figure S4. Oh et al. hTERT-HA Hdm2 1-542 - + 595-1132 1-350 350-1132 hTERT 115 90.5 46.2 61.5 37.8 IP: Hdm2 WCE 350-1132 hTERT 1132 1-350 1-542 595-1132 595 946 1 RT

a b c d e Supplementary Figure S5. Oh et al. 115 198 kDa 90.5 WB: HA WB: Hdm2 WB: Myc WCE 1 2 3 hTERT -(Ub)n IP: Myc + FLAG-Hdm2 HA-Ub Myc-hTERT MG132 - FLAG-Hdm2 hTERT-HA GFP - + WCE IP: FLAG FLAG-Hdm2 hTERT-HA - + - + + + + + WB: HA WB: FLAG 1 2 3 4 1 2 d e Mock1 Mock2 SC-7 SC-10 NC PC Mock-1 SC-7 SC-10 Mock-2 FLAG-Hdm2 Actin 50 bp 1 2 3 4 IC (36bp) 1 2 3 4 5 6

a b c Supplementary Figure S6. Oh et al. R1 R2 R1 R2 R1 R2 FLAG-negative R2 FLAG-positive R1 FLAG-negative R2 FLAG-positive R1 HA-negative R2 HA-positive