Lab 8 Muscle Physiology Gilbert R. Pitts, Ph.D., Joseph R. Schiller, Ph.D., and James F. Thompson, Ph.D.

Lab 8 Activities 1.Biopac Exercise L02-EMG-2: Skeletal Muscle Physiology

Multiple Motor Unit Recruitment

Biopac Exercises 1. Set Up 2. Calibration 3. Data Recording 4. Data Analysis

L02 - Electromyography (EMG) II Default: Record To Analyze later.

Set Up 2 1

Calibration With your dominant hand first, clench as hard as you can for 2 seconds; hit continue to start recording

Estimate Max Grip Force – note and then hit redo 48 kg (round to a number divisible by 4) First record the maximal force for your dominant hand and note the amount of force you generated in Kg (y axis) Hit suspend, and redo to collect the appropriate data

Multiple Motor Unit Recruitment (MMUR) 25% 50% 75% 100% not good, redo good Now you will clench at 25, 50, 75, and 100% of your maximal force; get a ~2 second plateau for each

Muscle Fatigue MMUR Fatigue Now, clench as hard as you can for as long as you can until you reach 50% of your maximal force, then let go

Complete Data Recording Next, repeat previous steps for your non-dominant arm

Record All Group Members Hit “ stop ” and then “ done ” Select “ record from another subject ” to record for the next group member; repeat until everyone has gone

First, print your graph: File> Print> Print Graph > Set Up> select “landscape” Under Display: 1.Autoscale Horizontal will “zoom out” to the entire data screen. 2.Autoscale Waveform to make the data fit the screen. Data Analysis

Data Analysis Screen Check that the channels are set correctly I-beam and zoom tools

Measuring MMUR on 4 Clenches Highlight the “plateau” of each clench on the clench force graph (blue line) and record clench data into table 1 on your lab report (page 8-9) Only highlight the flat portion for each clench (do not highlight the steep portions) highlight plateau

Measuring Fatigue on Continuous Clench Set 3 rd measurement box to channel 41 (Clench Force) and select “value” as the measurement (your lab manual says channel 1 – this is wrong) Click on the peak force (don’t highlight anything!) –Record the value of the clench force Highlight the continuous clench from the maximum force through 50% of the maximum and record data into table 2 on your lab report (page 8-9) 48 kg 24 kg highlight the range of 100% to 50% of your max clench force

Lab Report Assignment Write you instructor’s name as well as the day and time of your laboratory class on all sheets you turn in. Attach the graph you printed to the lab report sheet (page Lab 8-9) Mark and label on your data plot: 25% max, 50% max, 75% max, 100% max, and Muscle Fatigue for both the dominant and non-dominant arm Also label, with brackets, the dominant and non-dominant arm segments

Graph Labels 25% max 50% max 75% max 100% max Muscle Fatigue The labels go on the clench force graph (in the blue line) Dominant Arm Non-Dominant Arm 25% max 50% max 75% max 100% max Muscle Fatigue

End Lab 8 Presentation