1. The Respiratory System & Spirometry
Lab 6

2. Lab 6 Activities Identify parts of the respiratory system
Respiratory tree, thoracic organs, and larynx models
Fresh lung (if available)
Examine histology of the:
Trachea
Lung
Examine the operation of the lung model
Biopac L012-Pulmonary Function I
Measure lung volumes and capacities

3. Respiratory System Structure
Conduction zone: pathway for pulmonary ventilation
Respiratory zone: membrane for gas exchange  external respiration
Clinically, two parts:
Upper respiratory tract
Lower respiratory tract

4. Respiratory System Structure
Histology reflects the different functions of the different parts of the system
Position in respiratory pathway determines cell type
non-keratinized stratified squamous
pseudostratified ciliated columnar
cuboidal ciliated
simple squamous / Type I Alveolar cells

6. Pseudostratified Columnar Epithelium
mucosa
Lamina Propria
Submucosa
Hyaline Cartilage

7. seudostratified epithelium
= lamina propria
ucous glands
mooth muscle
artilage

8. Gross Section Through Lung

9. Smoker’s Lungs
Non-smoker 

10. Small Bronchiole

11. Lung Tissue slide Respiratory Bronchiole Alveolar Duct Alveoli
Alveolar Sac

12. Capillaries in Alveolar Wall

13. Spirometry

14. SPIROMETRY – RESPIRATORY VOLUMES AND CAPACITIES
Total Lung Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV) + Residual Volume (RV)
Vital Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV) + Expiratory Reserve Volume (ERV)
Inspiratory Capacity = Inspiratory Reserve Volume (IRV) + Tidal Volume (TV)
Functional Residual Capacity = Expiratory Reserve Volume (ERV) + Residual Volume (RV)

15. Spirometry: Calibration
Inlet
Inlet
Insert the calibration syringe/filter assembly into the side of the airflow transducer labeled "Inlet." 
Follow pop up window instructions
Wait for 8 seconds of no air flow,
Then do your 5 syringe cycles.

16. Experimental Treatments- Different from Biopac
Experimental Treatments- Different from Biopac!!!! and don’t forget the nose clip!!!
4 normal breaths (4 inhales/4 exhales)
Inhale as deeply as possible
Exhale to normal depth
3 normal breaths
Exhale as much as possible at end of
3rd exhalation
Inhale as much as possible, then exhale as fast and completely as possible (you will use this data segment in activity 3)
Click Stop- look at your recording and redo if necessary
If it’s good, select done and analyze your data.

17. BioPac Spirogram Example Data
If you get a negative value, just put the absolute value.
This part is in addition to what Biopac Lesson 12-Lung 1 prompts you to do!
1 regular exhale
1 strongest
inhale
3 regular
breaths
1 strongest
inhale
1 strongest exhale
3 regular
breaths
3 regular
breaths
1 strongest exhale

18. Biopac: Data Analysis Ch 2 P-P Measures Tidal Volume
Record in Table 1 on page Lab 6-17

19. Biopac: Data Analysis P-P Measures Inspiratory Capacity
Record in Table 1 on page Lab 6-17

20. Biopac: Data Analysis Delta Measures Inspiratory Reserve Volume
Record in Table 1 on page Lab 6-17
Use similar methods to determine:
Expiratory Reserve Volume
Vital Capacity

21. Biopac: Data Analysis (Activity 5)
Forced Expiratory Volume is the % of your forced vital capacity you can expel in
1,2, or 3 seconds.
RESET MEASUREMENT BOXES!!!!!
BOX 1- DELTA T BOX 2 P-P, ALL OTHER BOXES- NONE
USE I-beam to bracket one second of data starting at peak of last maximal
inhalation. P-P will give you FEV1 then highlight the 1st 2 seconds of data starting
at peak for FEV2 and then the 1st 3 seconds for FEV3
Record in Table 1 on page Lab 6-17

22. Biopac: Data Analysis
To measure FVC highlight the recording from just before last maximal inhalation to
just after peak of the last maximal exhalation- use P-P value.
Record in Table 1 on page Lab 6-19

23. Record your data in Excel at the computer located at the front podium
Summary of Class Data
Record your data in Excel at the computer located at the front podium
Your Instructor will make the complete data set available for you to use while completing the homework assignment

24. Homework Complete and turn in the questions on pages 6-17 to 6-20
Don’t forget to print the entire spirogram (landscape format) you recorded to include in your lab report.
Use vertical brackets ( [ ] ) to identify and label the tidal, inspiratory reserve, expiratory reserve and residual volumes; and total lung, vital, expiratory and inspiratory capacities on your spirogram.
Lab 6-19, Question 4. Remember to completely label your graphs!
Complete Assignment 6 on MasteringAandP.

25. The End
Lab 6

26. Biopac Spirometry
Residual volume

27. Spirometry