Quantification des structures 3D à partir de coupes histologiques sériées R. Kraftsik.

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Presentation transcript:

Quantification des structures 3D à partir de coupes histologiques sériées R. Kraftsik.

X Programmes disponibles : l Neurolucida - PC l structures arborescentes, coupes sériée l Volume- SGI Indigo + G.T. l coupes sériées

Neurolucida Neuron Tracing: Trace and analyze branching neuron processes. Brain Mapping: Map, count cells, and measure their locations and morphology Morphometry: Make accurate anatomical maps and measurements of histologically distinct structures and regions

Neurolucida system: -Neurolucida Software -Image Overlay Device (Optical and/or Video based) -Microscope Stage Control Device -Tracing Device -Graphics Output Device -PC Computer System.

Neurolucida system, room color camera 640 X stepping stage + controller - Zeiss microscope, light and fluorescence Contact: R. Kraftsik

FEATURES: - Accurately overlays a computer graphics or text display onto the microscope image - High brightness intensity - Up to 1024x668 pixels resolution (PC and MAC) - Accessible controls for contrast and brightness - Does not interfere with conventional use of the microscope Lucivid

DRG - CR Sciatic nerve

Volume Program, SGI Contact: R. Kraftsik

Volume program, SGI. Contact R. Kraftsik Reconstruction of cortical neurons from EM sections

Volume program, SGI. Contact R. Kraftsik Reconstruction of dendritic segments from EM sections

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