КОМПЬЮТЕРНЫЙ АНАЛИЗ КОНСЕРВАТИВНОСТИ АЛЬТЕРНАТИВНОГО СПЛАЙСИНГА В ОРТОЛОГИЧНЫХ ГЕНАХ Государственный научно-исследовательский институт генетики и селекции.

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КОМПЬЮТЕРНЫЙ АНАЛИЗ КОНСЕРВАТИВНОСТИ АЛЬТЕРНАТИВНОГО СПЛАЙСИНГА В ОРТОЛОГИЧНЫХ ГЕНАХ Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов (ГосНИИгенетика)

GENE organism 1 organism 2

GENE organism 1 organism 2

GENE exon 1exon 2 … exon 4 exon 3exon m-1exon m События в ортологичном гене : 1.консервация экзона 2.интронная вставка в экзон 3.делеция интрона 4.делеция экзона

isoform 1 isoform 2 GENE Классификация альтернатив 1. удержанный интрон

isoform 1 isoform 2 GENE 2. альтернативный донорный сайт Классификация альтернатив 1. удержанный интрон

isoform 1 isoform 2 GENE 2. альтернативный донорный сайт Классификация альтернатив 1. удержанный интрон 3. альтернативный акцепторный сайт

GENE isoform 1 isoform 2 2. альтернативный донорный сайт Классификация альтернатив 1. удержанный интрон 3. альтернативный акцепторный сайт 4. кассетный экзон

GENE isoform 1 isoform 2 2. альтернативный донорный сайт Классификация альтернатив 1. удержанный интрон 3. альтернативный акцепторный сайт 4. кассетный экзон 5. чередующийся экзон 1. внутренний Классификация экзонов и кодирующих сегментов 3. внешний isoform 1 isoform 2

coding segment 1 coding segment 2 … coding segment n-1 coding segment 3 coding segment n coding segment 4 isoform 1 isoform 2 isoform 3 isoform 4 GENE exon 1exon 2 … exon 4exon 3exon m-1exon m isoform 1isoform 2 … isoform k-1 isoform k coding segment 1 coding segment 2 coding segment 3 coding segment 4 coding segment 5 coding segment 6

Dme, Dps Aga

Dme, Dps Aga

Dps Aga Dme

Dme, Dps Aga stop (combined with acceptor site)

Dme, Dps Agaiiiiiiiv

Evolution of the exon-intron structure and alternative splicing in fruit flies and malarial mosquito genomes D.B.Malko, V.J.Makeev, A.A.Mironov, M.S.Gelfand Genome Research, 2006 Apr;16(4): Epub 2006 Mar 6.