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Gene Sizes Vary Strachan p146 DYSTROPHIN.

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Presentation on theme: "Gene Sizes Vary Strachan p146 DYSTROPHIN."— Presentation transcript:

1 Gene Sizes Vary Strachan p146 DYSTROPHIN

2 Multiple Small Exons are Typical
Strachan p191

3

4 Full-length first strand cDNA

5 Normalize

6 Normalize

7

8 Alternative Splicing Strachan p187

9

10 5’ RACE Miesfeld p153

11 Miesfeld p163 cont.

12

13 Short Splicing Signals
Brown (Genomes) p213

14 Finding Splice Sites C T G G T A A G T Exon Intron

15 100nt INTRON 1st EXON 1.5kb 800nt 5kb 150nt 150nt Last EXON Mid EXON 800nt

16 Short Polyadenylation signals
Brown (Genomes) 212

17 RNA Editing

18 MicroRNAs

19 Northern RNA blot Strachan p. 110

20 TaqMan Strachan p129

21 OLIGOS cDNAs

22

23

24 Sensitivity

25 RNASeq

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