BIO132 Lab 2 Exercise 29A/29 Blood

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Presentation transcript:

BIO132 Lab 2 Exercise 29A/29 Blood Human Blood Smear 40X objective (400X) Leukocyte Erythrocyte Platelet

Neutrophils 40X objective (400X)

Eosinophils 40X objective (400X)

Basophils 40X objective (400X)

Lymphocytes 40X objective (400X)

Monocytes 40X objective (400X)

Human Blood Smear 40X objective (400X) Leukocyte Erythrocyte Platelet

• Least dense component Formed elements Plasma • 55% of whole blood • Least dense component Buffy coat • Leukocytes and platelets • <1% of whole blood Erythrocytes Withdraw blood and place in tube. 1 Centrifuge the blood sample. 2 • 45% of whole blood (hematocrit) • Most dense component © 2013 Pearson Education, Inc.

X 100 Hematocrit = % blood occupied by cells Height of cells in mm Plasma Buffy Coat (WBCs + Platelets) Erythrocytes Air Clay Height of cells in mm Height of total in mm X 100 Expressed as % Typical = 45% Total Cells

Antigens present on Erythrocytes: Purified antibodies used to detect antigens: Antibody + Antigen = agglutination reaction (clumping)

Lymphatic Leukemia Normal Blood 40X objective (400X)

Normal Blood Sickle Cell Anemia 40X objective (400X)