Experiment 4 Thin Layer Chromatography. Silica gel (SiO2) glass or aluminum plate edge view.

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Presentation transcript:

Experiment 4 Thin Layer Chromatography

Silica gel (SiO2) glass or aluminum plate edge view

a b mix

solvent line starting point distance traveled by solvent distance traveled by solute r f = distance traveled by solute/distance traveled by solvent

It is the polar sites that are responsible for adsorbing solutes as they pass by. Depending on the polarity of the solute and adsorbent, some solutes are adsorbed more strongly than others. Adsorption is a competitive phenomena. The solvent as well as the solute is in competition for these active sites. If the solvent used is too polar, it will compete for these sites and preferentially bind to them leading to and R f > 0.8. R f = distance traveled by the solute/ distance traveled by the solvent R f values are always < 1 R f  0-0.2; solvent not polar enough R f  ; solvent too polar

Some solvents arranged in order of decreasing polarity going down and from left to right water acids (acetic acid, propionic acid) amides (N,N-dimethylformamide) alcohols (methanol, ethanol) amines (pyridine, trimethyl amine) ketones (acetone, 2-butanone, diethylketone) esters (methyl acetate, ethyl acetate) halides (chloroform, methylene chloride, carbon tetrachloride) ethers (tetrahydrofuran, dioxane, diethyl ether) aromatic hydrocarbons (benzene, toluene, xylenes) alkanes (hexane, heptane)