CHAPTER 8 Part 2 Cytoplasmic Membrane Systems: Structure, Function, and Membrane Trafficking Copyright © 2008 by John Wiley & Sons, Inc.

ER to the Golgi Complex: The first step in vesicular transport ERGIC Region Visualizing membrane traffic with a fluorescent tag

Vesicular Transport from the ER to the Golgi

The Golgi Complex

Glycosylation in the Golgi Complex No need to memorize the steps; only the concept is important

Dynamics of Transport through the Golgi Complex

COP1 and COPII Coated Vesicles

Vesicular Transport between membranous Compartments of the biosynthetic/secretory pathway

Roles of COPII proteins in assembling protein coat and capturing cargo

Accumulation of COPI- coated Vesicles

Retrieval of Resident ER Proteins !

Protein Transport from one Golgi cisternae to another can be assayed in a cell-free system (example: cultured fibroblasts); Fig a & b Fig. a

VSV G protein in vitro contains the additional N-acetylglucosamine!!! This modification is carried out by transferase enzyme that is moved by retrograde transport vesicles from the wild-type medial-Golgi cisternae to the mutant cis-Golgi cisternae in the reaction mixture (Science 276:1212, 1997). Fig. b

VERY IMPORTANT DIAGRAM ! Read Legend in Text-book; Fig 8.29 Targeting Lysosomal Enzymes To Lysosomes

Formation and Fusion of a Transport Vesicle

Formation of Calthrin coated Vesicles at the TGN

Model for Targeting of Transport Vesicles to Target Membranes

Model for interaction between v- and t-SNAREs leading to membrane fusion and exocytosis

LYSOSOMES

Lysosomes Liver Cells Neuron of a Human with Lysosomal Storage Disorder Accumulation of unidgested glycolipids