Determining Ruminant Materials In Feed Ingredients Larry L. Whitlock, Sr. Ph. D. Office of The Texas State Chemist College Station, Texas ASFFPCO Annual Meeting June 22-25, 2003 Fort Worth, Texas
Microscopy Equipment Compound Microscope Steroscopic Microscope Reagents for Spot Tests Accessories
Microscopic Procedures Use a 20 gram Sample for Viewing with the Stereomicroscope Take a Separate 10 gram Sample for Floatation Observe Mineral Portion Spot Tests for Confirmation
Spot Testing Reagants
Examples of Spot Testing 10% HC1 for Carbonates, Limestone, or Oyster Shell Nesslers Reagent for Ammonium Containing Compounds Urea Spot Testing Silver Nitrate for Chlorides Quimociac for Phosphates NOTE:Work with all floatation procedures in a hood to prevent breathing the vapors from the solvent
Application of Microscopy Levels of Meat and Bone in Feed Sample Identify Milk and Dried Blood Microscopy is a Rapid Procedure OTSC Current Surveys
Meat and Bone in Feed
Meat and Bone Meal in Feed
Bone Fragment
Animal Bone in Feed
Blood Meal in Feed
Meat and Bone Meal
Poultry Meal
Feather Meal
Hydrolyzed Feathers
Agri-Screen by Neogen Corporation Neogen has Two Test Strips Presence of Ruminant Meat and Bone in Animal Protein Products Presence of Ruminant Meat and Bone in Feeds
Agri-Screen Procedures for Ruminant M&B Meal Weigh 10 grams Sample in Mason Type Jar Add 100 ml Extraction Solution Place in Boiling Water Bath for 10 Minutes Remove Mason Jar from Water Bath Prepare Sample Tubes and Transfer 0.5 ml of Extract to a Tube (Use different pipette for each sample) Place Sample Strip into each Sample Tube Develop for 10 Minutes and Read Results
Reading Strip Results Negative-Control Line Appears Only Postive-Control Line and Sample Line Appear
Discussion