Unit 3 – Powerpoint #1 Honors Biology

 Use a beam of Light  The specimen can be dead or alive

 Have only 1 lens  Give two examples of a simple microscope: Magnifying Glass Reading Glasses

 Have more than one ______ lens

 Magnify by 10x or ______ or ________  Shine light on or ____________ the specimen  Specimen can be thick or _______. 20x 30x through thin

 Magnify by ______________  Shines light ______________ the specimen  Specimen must be _________ x thin through

 Use a beam of _______________-  The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________ electrons dead air water

 Makes 3D pictures of surfaces  Magnify by _________ 60,000x

 Makes _____ pictures of thin slices  Magnify by __________ 100,000x 2D

Butterfly Antenna

 Probe Microscopes  Ion Microscopes ◦ _____________ can be seen with these 2 types of microscopes. Atoms

 Horse Hair

1. Eyepiece Magnifies by 10x

2. Revolving Nose Piece Changes Objective Lens

3. Low power Objective Lens Magnifies by 4x (Red)

4. Medium Power Objective Lens Magnifies by 10x (yellow)

5. High Power Objective Lens Magnifies by 40x (Blue)

6. Stage Clips To hold slide in place

7. Diaphragm Adjusts the amount of light

8. Light Light to see specimen

9. On/Off Switch Turns light on and off

10. Arm To hold while carrying

11. Stage To set slide on

To focus only when in Low Power 12. Course Adjustment

13. Fine Adjustment To focus when using medium or high power

14. Base To hold while carrying

A. Carry a microscope with ___________ hands – one hand holding the _______ And the other hand under the ___________ Always hold the microscope upright so the _____________ cannot fall out When you place a microscope at your table, make sure it is _______ from the edge If there is anything wrong with the microscope, or it is damaged in any way, let the _______________ know immediately. B. To clean the objective lenses and eyepiece, use _______________________ BothArm Base Eyepiece Away Teacher Lens Tissue

1.Prepare your slide 2.Plug the microscope in. Turn on the ______________ 3.Be sure the ___________ Power Objective Lens (_______) is clicked into place. NOTE: Be sure to look at the microscope from the side when changing objective lenses to avoid damaging the ________________ and _____________ 4. Use coarse focus to raise the stage to its ________________ position 5. Place the _______________ on the stage. 6.Look through the eyepiece and turn the __________________ (lowering the stage) until the specimen is visible. 7. Use the ____________ adjustment to bring your specimen into sharp focus. You may wish to move the ________________ to adjust the amount of light. Light Low4X SlideLens Highest Slide Coarse Focus Fine Diaphragm

8. Make sure the specimen is ______________ 9. Switch to the ___________ Power Objective Lens ( ______) NOTE: Look at the microscope from the ____________ when you change lenses. 10. To avoid losing your focus and/or crushing the slide with the lens, never use the __________________ focus on medium or high power. Use the _____________ focus only to make the specimen visible. 11. Make sure the specimen is ______________. Look from the _________ as you change objective lenses and switch to the __________ Power Lens (________) 12. Use only the _____________ focus to make the specimen visible. 13. When finished, make sure the ___________ power objective lens is in place, wrap the __________ around the _________ of the microscope, cover, and place in the appropriate location Focused Mid10X Side Coarse Fine FocusedSide High40X Fine Low CordArm