F1 PRESS F1 FOR GUIDEANCE

Enterobacteria, Vibrio, Campylobacter & Pseudomonas Enterobacteria3 Station 1: Gram stain of Enterobacteria4 Station 2: NLF + LF on Mc Conkey5 Station 3: Klebsiella on Mc Conkey6 Station 4: E.coli on Mc Conkey7 Station 5: Pure LF & Pure NLF on both Mc Conkey & DCA8 Station 6: Proteus on Blood agar9 Station 7: Selenite F broth 10 Station 8: Indole production11 Station 9: H2S Production12 Station 10: Widal test13 Station 11: Treatment & diseases14 Vibrio15 Station 11: Gram stain of Vibrio cholera 16 Station 12: Growth on TCBS17 Station 13: Growth on TCBS & alkaline peptone water18 Campylobacter19 Station 14: Gram stain of Campylobacter20 Station 15: Urease Production21 Pseudomonas22 Station 16: Growth on Nutrient agar 23 Station 17: Sensitivity test24 Station 18: Growth on blood media25 Station 20: Treatment and diseases26

E.g. E.coli Klebsiella Proteus Shigella Salmonella and others. Non lactose fermenters Station 1

NLF= non lactose fermenters LF= lactose fermenters NLF LF Station 2

Large mucoid colonies (pinkish / reddish) Station 3

Pink colonies Station 4

Pinkish Pinkish Pale Pale LF (e.g. E-coli & Klepsilla) N.B. it is E-coli here “Shape of colonies” LF NLF (e.g. Salmonella, Shigella) NLF Station 5

Station 6

Samonella and Shigella Station 7

Station 8

Black e.g. shigella Station 9

1/10 1/20 1/40 ……… 1/640 + test - test 1/10 1/20 1 / /10 1/20 1/40 1/80 1/160 / There is more than 4 folds increase in the titer of antibodies against Salmonella typhi “320/40= 8”, this means acute infection. Station 10

Escherichis coli Infection:- 1- urinary tract infection 2- diarrhea Treatment:- requires antibiotic testing, but most common are 1-”Ampicilin” 2-”Aminglycosides” Klebsiella Infection:- urinary tract infection Treatment:- beta lactamase producing “Aminglycosides” is used Proteus Infection:- urinary tract infection, by the urease the urea is split to produce ammonia  “alkaline urine” Salmonella Infection:- 1- typhoid fever 2- food poising “meat, poultry & egg”  diarrhea This usually happens when people eat in public places “Pakistan, Sudan & Egypt” Treatment:- In enteric fever  “chloramphenicol”, if resistant  “ciprofloxacilin” Shigilla Infection:- dysenteriae (enterotoxin, cytotoxic & neurotoxic)  bloody, mucoid & small stools with fever Treatment:- Fluid replacement & “Ampicillin” Station 11

E.g. V VV Vibrio Cholerae Station 12

Station 13

aureus Mentioned previously Clear Turbid Station 14

(vibrio) E.g. C CC Campylobacter jejuni Station 15

Helicobacter pylori Station 16

No pigment Station 17

Sensitive Sensitive Pseudomonas is resistant to many antibiotics “but sensitive to amino glycosides (e.g. gentamicin) and some others” Station 18

Note that Pseudomonas is strictly aerobic Greenorange Station 19

Vibrio cholera Infection:- cholera (exotoxin  persistent and excessive secretion of isotonic fluid by intestinal mucosa) Treatment:- “Erythromycin” Campylobacter jejuni Infection:- Food poisoning “Chicken”  diarrhea. Treatment:- “Erythromycin” Helicobacter pylori Infection:- Gastric and duodenal ulcerations Treatment:- Triple therapy: “Omeprazole” + “Amoxycilin” + “Metronidazole” Quadruple therapy: Same + proton pump inhibitors Pseudomonas Infection:- Gastric and duodenal ulcerations Treatment:- “Erythromycin”, “Amoxycilin” Station 20

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