1. PRESS F1 FOR GUIDEANCE
Bacteriology
5th Practical
MFSH 2003

2. Contents Haemophilus, Mycobactria , Corynbacteriaua & Bordetella
Station 1: Gram stain of Haemophilus 4
Station 2: Gram stain of Haemophilus (pus) 5
Station 3: X,V factors & growth
Station 4: Growth of H.Influenzae on agar 7
Station 5: Satellitism
Station 6: Treatment & diseases
Station 7: Summary
Mycobacteria
Station 8: Ziehl-Neelsen stain
Station 9: Lowenstien Jensen media
Station 10: Treatment & diseases
Station 11: Summary
Cornybacteria
Station 12: Gram stain of Cornybacteria
Station 13: Albert stain of Cornybacteria
Station 14: Media used
Station 15: ELEK’s plate
Station 16: Treatment & diseases
Station 17: Summary
Bordetella
Station 18: Laboratory diagnosis
Station 19: Tratment & diseases

3. Haemophilus

4. It can look like Bacillus or may look like coccus
Station 1
It can look like Bacillus or may look like coccus
Coccobacilli

5. Station 2

6. Some of them require X & V factors ( Haemophilus influenzae)
Station 3
Different species of “Haemophilus” have different requirements to grow and make colonies.
Some of them require X & V factors ( Haemophilus influenzae)
Some of them need X factor only (Haemophilus ducreyi)
Some of them need V factor only (Haemophilus parainfluenzae)
X factor = heamatin
V factor = Nicotinamide, Adenine & Dineiceotide
This Bacteria grow when there are V & X factors. E.g. (H. Influenzae)
This Bacteria grow when there is V factor. E.g. (H. Parainfluenzae)

7. Station 4
Only X factor
X+V factors
No growth
Growth
No growth

8. Station 5 X Factor present This is called Satellitism
Staphylococcus strip
Producing V Factor
H.Influenzae growing here because of presence of both X & V factors

9. Station 6 Haemophilus influenzae Infection:-
respiratory tract infections
No bacteremia
Haemophilus influenzae
Infection:-
meningitis
acute epiglotitis in children (sore throught)
osteomylitis & arthritis
Cause bacteremia
Treatment:-
“Ampicilin”
if beta lactamase producers  “Chloramphenicol”
Vaccination:-
Present by Polysaccharide capsule “serotype b”

10. Grows on Chocolate agar
Station 7
Summary
Test
yourself H.
influenzae H.
Parainfluenzae
Gram stain
Type
Needs (X,V factors)
Grows on Chocolate agar
Grows on Blood agar
-ve -ve
Coccobacilli Coccobacilli
X + V V only
Yes Yes
No No
Best wishes

11. Mycobacteria

12. Station 8

13. Station 9

14. Station 10 Mycobacterium tuberculosis Infection:-
1-TB “lung infection with bloody cough”
2- Meningitis
Treatment:-
“Pyrazinamide + Isoniazid + Rifampicin”  2 months
then,
“Isoniazid + Rifampicin”  4 months
Vaccination:-
B.C.G “Bacille-Calmatte Guerin “10 years protection”

15. Summary Best wishes Type “of Mycobacterium”: Stain used:
Station 11
Summary
Type “of Mycobacterium”:
Stain used:
Medium used:
Acid-Fast Bacilli
Ziehl-Neelsen Stain
Lowenstein-Jensen medium
Best wishes

16. Corynebacteria

17. Station 12

18. Station 13

19. Black color Station 14 Something like this
There is growth but this is a wrong plate

20. Station 15

21. Station 16 Corynbacterium diphtheriae Infection:-
Diphtheria “Bull neck appearance because of pharyngitis”
affect: 1- nervous tissue damage (exotoxin)
2- heart muscle damage (exotoxin)
3- laryngeal obstruction
4- affect skin
No bacteremia
Treatment:-
“Penecilin”
Vaccination:-
Present by toxoid “DPT vaccine”
*D= diptheria toxoid
*P= pertussis
*T= tetanus

22. Summary Best wishes Type & “gram” stain: Stains used: Gram +ve bacilli
Station 17
Summary
Type & “gram” stain:
Stains used:
Media used:
What is used for toxogenity test?
Gram +ve bacilli
Gram & Albert
Potassium tellurite & Loeffler’s
ELEK’s plate
Best wishes

23. Bordetella

24. Station 18
(coocobacili)

25. Station 19 Bordetella pertussis Infection:-
Whooping cough “due to an endotoxin” usually 1 year of age
complications: bronchopneumonia, bronchiatasis & subconjuctival hemorrhage
Treatment:-
“Erythromycin” but only affective at early stages
if beta lactamase producers  “Chloramphenicol”
No bacteremia
Vaccination:-
Present by whole killed bacteria “DPT”

26. اللهم إنا نسألك زيادة في الدين وبركة في العمر وصحة في الجسد
وسعة في الرزق
وتوبة قبل الموت
وشهادة عند الموت
ومغفرة بعد الموت
وعفوا عند الحساب
وأمانا من العذاب
ونصيبا من الجنة
وارزقنا النظر إلى وجهك الكريم
دعواتكم يا شباب

27. I hope it was useful.
A word can change the outcome of many things. So, if you either liked or didn’t like this project, why keep your thoughts to yourself ?
Send us your comments and ideas to It will mean too much to us, “even if it was just one word”
THANX
M. F. Shaheen 2003