Rev B 09/2012 ChromoPlex 1 Dual Detection Multiply Your Capabilities Leica ChromoPlex TM 1 Dual Detection for Leica BOND

Rev B 09/2012 ChromoPlex 1 Dual Detection Double Staining Just Became Easy Leica introduces a new range of detection systems for Leica BOND – ChromoPlex TM

Rev B 09/2012 ChromoPlex 1 Dual Detection Double Staining Just Became Easy This new range of multiplex detection systems increases Leica BOND productivity, and demonstrates Leica’s commitment to lead in the provision of quality focused detection systems

Rev B 09/2012 ChromoPlex 1 Dual Detection Double Staining Just Became Easy ChromoPlex TM 1 Dual Detection is the first detection system to be launched in this new range

Rev B 09/2012 ChromoPlex 1 Dual Detection  Is a Dual Polymer Detection System with a rapid 1-step staining protocol Double Staining Just Became Easy Leica ChromoPlex 1 Dual Detection for Leica BOND

Rev B 09/2012 ChromoPlex 1 Dual Detection  Detects primary antibody cocktails (Mouse/Rabbit), both self validated and commercially manufactured Leica ChromoPlex 1 Dual Detection for Leica BOND Double Staining Just Became Easy

Rev B 09/2012 ChromoPlex 1 Dual Detection  Allows visualization of different epitopes with two different colored chromogens on the same slide Double Staining Just Became Easy Leica ChromoPlex 1 Dual Detection for Leica BOND

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Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with excellent detail Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative Multiply your capabilities with  Excellent detail of tissue morphology with red and brown chromogens Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with multiple antibodies Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Insight for diagnosis with multiple antibodies on the same slide Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with Clear differentiation Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Clear color differentiation Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with antibody cocktails Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Disease specific antibody cocktails as clinical needs dictate Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with reduction in turnaround time Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection When tissue is limited, the most effective use of tissue sections becomes imperative. Multiply your capabilities with  Perform dual staining within a day’s work with significant reduction in turnaround time Menu Multiply Your Capabilities

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Clear Differentiation Flexibility Complete The Picture With Leica ChromoPlex 1 Dual Detection for Leica BOND, you can MULTIPLY YOUR CAPABILITIES >  Make comparative diagnostics easier Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner. Menu

Rev B 09/2012 ChromoPlex 1 Dual Detection Clear Differentiation Flexibility Complete The Picture With Leica ChromoPlex 1 Dual Detection for Leica BOND, you can MULTIPLY YOUR CAPABILITIES >  Lead the way in diagnostic efficiency Menu Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Clear Differentiation Flexibility Complete The Picture With Leica ChromoPlex 1 Dual Detection for Leica BOND, you can MULTIPLY YOUR CAPABILITIES >  Improve the opportunity for same day diagnostics Menu Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Make Double Staining Routine Practice “the dual staining obtained using the ChromoPlex 1 detection system is attractive, crisp and consistent; ideal when assessing challenging prostatic biopsies.” Dr Geoffrey Hulman MD FRCPath Nottingham University Hospitals NHS Trust

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Make Double Staining Routine Practice For the Pathologist who wants to make the most effective use of tissue from core biopsies and small tissue sections, Leica ChromoPlex 1 Dual Detection for Leica BOND will significantly increase the opportunity for same day diagnosis. ChromoPlex is a fully automated IHC system delivering dual chromogen staining in a rapid, one-step process “the dual staining obtained using the ChromoPlex 1 detection system is attractive, crisp and consistent; ideal when assessing challenging prostatic biopsies.” Dr Geoffrey Hulman MD FRCPath, Nottingham University Hospitals NHS Trust

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Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Maximize workflow Efficiency Now you have the ability to fully automate parallel dual chromogen staining using ChromoPlex 1 Dual Detection for Leica BOND

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection 1-step parallel staining Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection  1-step parallel staining that reduces turnaround time compared to 2-step sequential staining methods Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection one detection system Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection  One detection system, 2 color chromogens Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection validated protocol Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection  Fully automated, validated protocol Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Leica BOND Covertile™ technology Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection  Leica BOND Covertile TM technology protects tissue and minimizes damage Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection same day slide delivery Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection  Improve your opportunity for same day slide delivery for diagnosis Maximize workflow Efficiency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can  Simplify staff training Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can  Reduce the number of slides requiring staining Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can  Increase flexibility to accommodate additional validated staining protocols Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection MAXIMIZE WORKFLOW EFFICIENCY > Increase Efficiency Save Time Expand Capacity With the ability to now fully automate parallel dual chromogen staining using Leica ChromoPlex 1 Dual Detection for Leica BOND, you can Provide rapid service to Pathologists Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Uninterrupted processing and total automation with Leica BOND and ChromoPlex 1 Dual Detection frees up staff and facilitates effective resource optimization MAXIMIZE WORKFLOW EFFICIENCY > Implement Double Staining on Leica BOND Comparison of automated staining methods Leica BOND Polymer Refine – Single Staining DewaxRetrievalPeroxide BlockAntibodyPost PrimaryPolymerDABHematoxylin Leica ChromoPlex 1 Dual Detection for Leica BOND DewaxRetrievalPeroxide Block Antibody Cocktail Polymer mHPR Polymer rAP DAB Red Chromogen Hematoxylin Single staining Parallel Dual Chromogen staining 165 minutes* 42 minutes* *reference? *Based on 10 slides x 1 SSA. Actual time dependent on sample set-up

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Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency With the already well established Compact Polymer technology, Leica ChromoPlex 1 Dual Detection for Leica BOND delivers the quality and performance that you associate with Novocastra TM - results that can be relied upon consistently

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection intense staining Ensure Consistency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection  Intense high resolution staining Ensure Consistency with

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with enhanced tissue penetration

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Enhanced tissue penetration through the use of small multifunctional linkers

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with excellent contrast

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Excellent contrast between Fast Red and DAB chromogens

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with color differentiation

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Easy color differentiation

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with consistent staining

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Ensure Consistency with  Uniform reagent application which facilitates clear, consistent differentiated antigen staining

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection ENSURE CONSISTENCY > Quality Sensitivity Specificity Leica ChromoPlex 1 Dual Detection for Leica BOND delivers the same quality and performance that you associate with Novocastra products, with  Two chromogens in one detection system  Intense staining with a clean background  Multiple antibodies on a single slide User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB). Digital image captured using the Leica SCN400 Slide Scanner.

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Successfully Implement Double Staining “Historically, we encountered problems with dual staining and efforts to stain with commercial cocktails have been largely unsuccessful. The Leica ChromoPlex 1 detection system, however, enables quick easy staining of both commercial and home-brew cocktails, with excellent results.” Dr Claire Hawkes PhD, CSci Nottingham University Hospitals NHS Trust ENSURE CONSISTENCY >

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Successfully Implement Double Staining Leica ChromoPlex 1 Dual Detection for Leica BOND delivers quality, sensitivity and specificity providing you with results that can be relied upon consistently “Historically, we encountered problems with dual staining and efforts to stain with commercial cocktails have been largely unsuccessful. The Leica ChromoPlex 1 detection system, however, enables quick easy staining of both commercial and home-brew cocktails, with excellent results.” Dr Claire Hawkes PhD, CSci - Nottingham University Hospitals NHS Trust ENSURE CONSISTENCY >

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Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Staining Gallery Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner CLICK A PICTURE TO ENLARGE > Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner Antibody Cocktail - Ki67 (red) and CD3 (DAB) on Human Tonsil Digital image captured using the Leica SCN400 Slide Scanner User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB) Digital image captured using the Leica SCN400 Slide Scanner

Gallery Rev B 09/2012 ChromoPlex 1 Dual Detection User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB) Digital image captured using the Leica SCN400 Slide Scanner

Gallery Rev B 09/2012 ChromoPlex 1 Dual Detection Antibody Cocktail - Ki67 (red) and CD3 (DAB) on Human Tonsil Digital image captured using the Leica SCN400 Slide Scanner

Gallery Rev B 09/2012 ChromoPlex 1 Dual Detection Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner

Gallery Rev B 09/2012 ChromoPlex 1 Dual Detection Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner

Menu Rev B 09/2012 ChromoPlex 1 Dual Detection Staining Gallery Biocare ADH-5 TM (Breast Marker Cocktail) – CK7/18 (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner CLICK A PICTURE TO ENLARGE > Biocare PIN-4 ® (Prostate Marker Cocktail) – P504S (Red) and CK5/14 and p63 (DAB) Digital image captured using the Leica SCN400 Slide Scanner Antibody Cocktail - Ki67 (red) and CD3 (DAB) on Human Tonsil Digital image captured using the Leica SCN400 Slide Scanner User validated Prostate Marker Cocktail – P504S (Red) and HMW CK (DAB) Digital image captured using the Leica SCN400 Slide Scanner

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Thank you Leica ChromoPlex TM 1 Dual Detection for Leica BOND Multiply Your Capabilities