Salk Institute Mobile Lab Gel Electrophoresis Student Instructions VWR Set up.

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Presentation transcript:

Salk Institute Mobile Lab Gel Electrophoresis Student Instructions VWR Set up

Salk Institute Mobile Lab Gel Electrophoresis Student Station Set-up tip container w/tips waste container Micro-pipettor practice sheet in protector foam epi rack w/ 1 food color tube each: orange, green, purple

Salk Institute Mobile Lab How to Use a micro-pipettor Parts of a Micro-pipettor –Make sure a yellow tip is firmly twisted onto the end of the micro-pipettor –Adjust the dispense amount to 5 µl (05.0) by turning the knob at the top Plunger Amount window Eject button Barrel Yellow tip

Salk Institute Mobile Lab How to Use a micro-pipettor Carefully open one of the food color tubes –Don’t let the lid snap back and splash you! To Fill – press down gently with your thumb until you feel the plunger stop. This is STOP 1. While holding the plunger at Stop 1 place the tip into the liquid and release the plunger. This will draw up the precise volume you want.

Salk Institute Mobile Lab How to Use a micro-pipettor To Dispense – press down hard on the plunger, you’ll pass Stop 1 and continue to STOP 2. This will fully eject the volume from the tip. Using your other hand to guide the end of the tip, practice dispensing the 5µl drops –Try to not push down on the plastic sheet - see if you can hold the pipettor tip just above the paper

Salk Institute Mobile Lab How to Use a micro-pipettor Push green button to eject the dirty tip into the waste container and replace with a clean tip Always use a clean tip each time!!

Salk Institute Mobile Lab How to Use a micro-pipettor Ready to load a real gel?

Salk Institute Mobile Lab Loading the gel Load the food color solutions into the wells on your group’s gel Be careful not to poke through the bottom of the wells!