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Do Now How many ul (microliters) are in one liter?

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Presentation on theme: "Do Now How many ul (microliters) are in one liter?"— Presentation transcript:

1 Do Now How many ul (microliters) are in one liter?

2 Objectives 1. SWBAT practice using the micropipette.
2. SWBAT measure out 20 ul, 10 ul, 5 ul, and 2ul.

3 Micropipetting Three types: Very expensive instrument for measuring
*** A set of 3 (p20, p200, p1000) can range from $750- $1,000***** Measures very small volumes: microliters (ul) _________ microliters = 1 liter Three types: P __________ - __________ P-200 __________ - __________ P-20 __________ - __________

4 Micropipette What is the volume of this pipette? 2 20 ul

5 Reading the p20:

6 Parts of the Micropipette
Tip Plunger Adjustment dial Volume window

7 Using the Micropipette
Determine the volume to be pipette. Rotate the dial to the appropriate setting. NEVER GO ABOVE THE MAXIMUM VOLUME THAT IS MADE FOR THE MICROPIPETTE Use a clean pipette tip from box. Gently push micropipette onto tip. USE PROPER STERILE TECHNIQUE To draw up solution – depress plunger to first stop; release plunger and draw up solution; place tip at bottom of tube; depress plunger to second stop. Eject the used tip over and into the waste basket on table.

8 Holding the Pipette WSSP Lab Video


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