1. Using the micropipette
Biotech Lab #5
Using the micropipette
Do you remember?
What is the metric unit for volume?
Can you list metric prefixes which make the unit smaller?

2. Micropipette:

3. Micropipetting Three types: Very expensive instrument for measuring
*** A set of 3 (p20, p200, p1000) can range from $750- $1,000*****
Measures very small volumes: microliters (ul)
_________ microliters = 1 liter
Three types:
P __________ - __________
P-200 __________ - __________
P-20 __________ - __________

4. Micropipette
What is the volume of this pipette? 2
20 ul

5. Reading the p20:

6. Parts of the Micropipette
Tip
Plunger
Adjustment dial
Volume window

7. Using the Micropipette
Determine the volume to be pipette. Rotate the dial to the appropriate setting. NEVER GO ABOVE THE MAXIMUM VOLUME THAT IS MADE FOR THE MICROPIPETTE
Use a clean pipette tip from box. Gently push micropipette onto tip.
USE PROPER STERILE TECHNIQUE
To draw up solution – depress plunger to first stop; release plunger and draw up solution; place tip at bottom of tube; depress plunger to second stop.
Eject the used tip over and into the waste basket on table.

8. Holding the Pipette
WSSP Lab Video