Microscope Basics T. Trimpe 2005 http://sciencespot.net/

Body Tube Stage Stage Clips Coarse Adjustment Light Always carry a microscope with one hand holding the arm and one hand under the base.

Fill in the table on your worksheet. What’s my power? To calculate the power of magnification, ________the _______of the _____________by the _________of the __________. What are the powers of magnification for each of the objectives we have on our microscopes? Fill in the table on your worksheet.

Comparing Powers of Magnification We can see better details with higher the powers of magnification, but we cannot see as much of the image. Which of these images would be viewed at a higher power of magnification?

Parts of a Micro-scope

GIVE ME A LABEL AND COLOR! __________Lines pointing to specimen Nucleus Cell Wall ____________what you are identifying Label: Onion Plant Cell Mitosis Magnification: 100X

Calculating Objective Magnification Magnification of Eyepiece = ____x Magnification of Objective = 4X, _____, or ______ (10X) (____) = _____x magnification!

Microscopes Surgical Scope Dissection Scope

The Microscope Rules of Handling: Carry using __________hands. Place it towards the ___________of the table. Keep the cord out of the way. Rotate the objective so the ___________objective is set to the stage. Turn ________the ____________. Unplug & wind up the cord before returning to the ____________.

Types of Microscopes Electron (use beams of electrons) Magnify 1000 – 2200 x or more Can’t be used on living organisms Transmission (TEM) for thin flat samples Scanning (SEM) for 3-D details Light Microscopes (focus visible light) Magnify 100 - 1000 x Can be used on living organisms Simple (1 lens) Compound (2 lenses)

Let’s give it a try ... 1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it! 4 – When you are done, turn off the microscope and rotate so the lowest objective is facing the slide. Put AWAY the slides you used.

You do not need to use the stage clips when viewing wet-mount slides! How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.