1. Microscope
Basics
T. Trimpe

2. Ocular lens (Eyepiece)
Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Always carry a microscope with one hand holding the arm and one hand under the base.
Base

3. Fill in the table on your worksheet.
What’s my power?
To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective.
What are the powers of magnification for each of the objectives we have on our microscopes?
Fill in the table on your worksheet.

4. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

5. Let’s give it a try ...
1 – Turn on the microscope and then rotate the nosepiece to click the scanning objective into place.
2 – Place a slide on the stage . Use your fingers to position the slide on top of the light in order to view your cell. Use the stage clips to clip slide in place. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet!
DO NOT USE COARSE ADJUSTMENT ON HIGH POWER
4 – When you are done, turn off the microscope, unplug it and wrap cord around the base. Cover with the dust cover. Return all materials.

6. How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place tissue to be viewed in middle of slide. Place ONE drop of water on top of tissue. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
5 – Place the slide on the stage and view it first with the scanning objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

7. First to View Cells
In 1665, Robert Hooke used a microscope to examine a thin slice of cork (dead plant cell walls)
What he saw looked like small boxes
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8. First to View Cells Hooke is responsible for naming cells
Hooke called them “CELLS” because they looked like the small rooms that monks lived in called Cells
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9. Anton van Leeuwenhoek “Father of Microscopy”
In 1673, Leeuwenhoek (a Dutch microscope maker), was first to view organism (living things)
Leeuwenhoek used a simple, handheld microscope to view pond water & scrapings from his teeth
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10. Hans and Zacharias Janssen
In 1590, Hans and Zacarias Janssen ( Dutch microscope makers), were first to invent a compound microscope (two lenses in a tube)
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