1. What is this and what is it used for?

2. T. Trimpe 2005 http://sciencespot.net/

3. Body Tube or barrel Nosepiece Objective lenses Stage Clips Light Eyepiece Arm Stage Coarse Adjustment Fine Adjustment Always carry a microscope with one hand holding the arm and one hand under the base. Base Diaphragm

4. What’s my power? To calculate the power of magnification, multiply the power of the eyepiece by the power of the objective lens. What are the powers of magnification for each of the objectives we have on our microscopes? Fill in the table on your worksheet.

5. Band Color Objective Power Eyepiece Lens Power Power of Magnification Red Yellow Blue

6. Comparing Powers of Magnification We can see better details with higher powers of magnification, but we can’t see as much of the specimen. Which of these images would be viewed at a higher power of magnification?

7. Let’s give it a try... 1 – Turn on the microscope and then rotate the nosepiece to click the red-banded objective lens into place. 2 – Place a slide on the stage and secure it using the stage clips. Use the coarse adjustment knob (large knob) to get it the image into view and then use the fine adjustment knob (small knob) to make it clearer. 4 – When you are done, turn off the microscope and take off the slide you used. 3 – Once you have the image in view, rotate the nosepiece to view it under different powers. Draw what you see on your worksheet! Be careful with the largest objective! Sometimes there is not enough room and you will not be able to use it!

8. Gather the Following Materials… Pipette (1 per pair) Water sample (1 per table) Paper Towel Slide + Cover slip “Kimwipe”

9. How do you make a wet-mount slide? … 1 – Get a clean slide and coverslip from your teacher. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop. You do not need to use the stage clips when viewing wet-mount slides! 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. 4 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly

10. Microscope Mania! (half sheet of paper) You will examine 2 specimens under the microscope and draw what you see. Remember to include the name of your specimen and the total magnification you viewed the specimen under. Specimen 1: Leaf 1.Place the leaf cutting on a dry, clear slide (see below). 2.Remember to start with the smallest (red) objective!! 3.Clean and wipe your slide when finished. Deposit your specimen in the trash can. Specimen 2: Your Choice! 1.Choose something in the classroom to view under the microscope and follow the same procedure. 2.Remember to clean up when you are finished!

11. Exit Ticket – back of drawings Answer the following questions (complete sentences!): 1.Describe any problems you had using the microscope. 2.What do you now understand about cells/microscopes that you did not before? 3.How did you use your imagination/creativity today?