Species tree phylogenetics of a clade of Cuban Anoles Seth Rudman.

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Presentation transcript:

Species tree phylogenetics of a clade of Cuban Anoles Seth Rudman

Introduction to Anolis Anolis sagrei, one of Cuba's most common species; Anolis vermiculatus, an aquatic species from western Cuba; Anolis barbatus, a strange Cuban species that feeds on snails; Anolis allisoni, another Cuban species; Anolis bartschi, a spectacular rock- dweller that lacks a dewlap; Anolis distichus, from Hispaniola; Anolis aliniger, a montane species from Hispaniola (center).

Introduction to Anolis Anolis sagrei, one of Cuba's most common species; Anolis vermiculatus, an aquatic species from western Cuba; Anolis barbatus, a strange Cuban species that feeds on snails; Anolis allisoni, another Cuban species; Anolis bartschi, a spectacular rock- dweller that lacks a dewlap; Anolis distichus, from Hispaniola; Anolis aliniger, a montane species from Hispaniola (center).

Ecomorphs

Anolis sagrei

Phylogenetics basics: a quick review

Making a Gene Tree 1) Collect tissue 2) Extract DNA and Sequence 1 mtDNA marker and 2 common nDNA markers

3) Align sequences and make a tree

Difficulties of Phylogenetics Conflicting gene histories Gene A reconstructs this topologyGene B reconstructs this topology

Difficulties of Phylogenetics Short distances between nodes

Problems encountered by my early trees…

Species Trees Helpful to imagine a gene tree as a tree contained within the true phylogeny. Think of a species tree as an aggregate of all gene trees.

What I’m doing now Re-sample characters to form smaller datasets and determine the importance of sampling many nuclear loci.