The case of the “wondering” goat Ronald D. Tyler Jr.* Geoff Saunders* Bernard Jortner* Dan Righter † James Mandell‡ Gerald Campbell § * Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg, VA † Washington Animal Disease Diagnostic Laboratory, Pullman, WA ‡University of Virginia Medical Center, Charlottesville, VA §University of Texas Medical Center, Galveston, TX Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Signalment -“Streak” -11-year-old Alpine- Toggenburg cross wether Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

History Goat has been “wondering” in pasture for past month. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

History - really Goat has been “ wandering ” in pasture for past month & star gazing. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Gross Pathology No gross lesions were initially noted at necropsy. During sectioning of the brain a well demarcated mass was discovered in the mid-brain extending to the rostral brainstem. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Pineal gland mons/6/6b/Illu_pituitary_pineal_glands.jpg Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Histopathology: Brain: Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Immunohistochemistry Cytokeratin = Positive GFAP = Negative Synaptophysin = Positive S-100 = Positive Neurofilament = Negative –βIII-tubulin (mAb TUJ1) = Pending –S-Antigen = Pending Synaptophysin IHC Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Immunohistochemical profiles: PNET (neuroblastoma)Pineocytoma CytokeratinPositive SynaptophysinPositive Neurofilament proteinPositive GFAPNegative NSEPositive S-100Positive class III ß-tubulinPositiveNegative S-AntigenNegativePositive Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Distinguishing features PineocytomaNeuroblastoma (PNET) Histological characteristics Uniform cells that tend to form "Pineocytomatous rosettes" which are large zones of fine fibrilar processes surrounded by hyperchromatic oval nuclei. The mitotic rate is generally low. 6 Uniform cells that tend to palisade and form pseudorosettes and/or Homer-Wright rosettes. Neoplastic nuclei are hyperchromatic nuclei and mitotic figures are common. There are occasional regions of dystrophic mineralization. 6 Similarities Similar to medulloblastomas (reserved for neurocytic tumors of the cerebellum) 1 Ultrastructural characteristics (Electron Microscopy) Cytoplasmic microtubules, dense core vesicles and possibly cilia with a 9+0 configuration. 4,5 Cytoplasmic dense-core neurosecretory granules, microtubules and synapse like structures. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Rosettes Homer-Wright rosettes: Supratentorial PNET (Neuroblastoma) Pineocytomatous rosettes: Pineocytoma Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Electron Microscopy Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Case Summary Differential Diagnoses: - Pineocytoma - PNET (Neuroblastoma) Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

References 1.Maxie MG, Youssef S: Neoplastic Diseases of the Nervous System. In: Jubb, Kennedy, and Palmer’s Pathology of Domestic Animals, ed. Maxie MG, 5 th ed., v1., pp , Elsevier-Mosby, St. Louis, MO, Koestner A, Higgins RJ: Tumors of the Nervous System. In: Tumors in Domestic Animals, ed. Mueten DJ, 4 th ed., pp , Iowa State Press, IA Summers BA, Cummings JF, DeLahunta A: Tumors of the central nervous system. In: Veterinary Neuropathology, ed. Summers BA, Cummings JF, DeLahunta A., pp , Mosby, St. Louis, MO, Dario A, Cerati M, Taborelli M, Finzi G, Pozzi M, Dorizzi A: Cytogenetic and ultrastructural study of a pineocytoma, case report. Journal of Neuro- Oncology v48: pp , Fevre-Montange M, Jouvet A, Privat K, Korf HW, Champier J, Reboul A, Auera M, Mottolese C: Immunohistochemical, ultrastructural, biochemical and in vitro studies of a pineocytoma. Acta Neuropathology. v95: pp , Ellison D, Love S: Neoplasms. In: Neuropathology, ed. Ellison D, Love S, 2 nd ed., pp , Mosby, St. Louis, MO, 2004 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

Acknowledgments & Co-Authors Special thanks to the many consultants on this case for lending there time and expertise. Dr. Ronald D. Tyler Jr., Virginia Maryland Regional College of Veterinary Medicine, Anatomic Path Resident Dr. Geoff Saunders, Virginia Maryland Regional College of Veterinary Medicine, Anatomic Pathologist Dr. Bernard Jortner, Virginia Maryland Regional College of Veterinary Medicine, Anatomic Pathologist Dr. Dan Righter, Washington Animal Disease Diagnostic Laboratory, Anatomic Path Resident Dr. James Mandell, University of Virginia Medical Center, Department of Pathology, Human Neuropathologist Dr. Gerald Campbell, University of Texas Medical Center, Department of Pathology, Human Neuropathologist Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

The End Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only