1. Pituitary Pars Intermedia Dysfunction (PPID) Bonnie Brenseke, DVM Anatomic Pathology Resident Virginia Maryland Regional College of Veterinary Medicine Authors: Bonnie Brenseke, Tanya LeRoith, and Geoff Saunders Contributors: Mark Crisman and Jamie Wearn Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

2. “ Kibbles ” *not really “Kibbles”, hoofcare.blogspot.com Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

3. For the washer/dryer and free trip to Hawaii… Classic case of PPID/Equine Cushing’s Syndrome Common endocrine disorder of aged horses and ponies Etiology and pathophysiology? -primary lesion of the pituitary gland -loss of dopaminergic inhibition on the pars intermedia www.hawaiiguide.com www.saatchi- gallery.co.uk Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

4. Anatomy: Banks, William. Applied Veterinary Histology. Baltimore, MD: Williams & Wilkins, 1981. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

5. Pathogenesis: Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

6. Clinical Signs  due to hormone dysregulation & to space- occupying effects Hypertrichosis (Hirsutism)- almost pathognomonic Hyperhydrosis Laminitis Polyuria/Polydipsia Polyphagia ↑’ed susceptibility to infections (skin, lung, parasites) Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

7. Clinical signs cont’d Behavioral changes- docility, lethargy, narcolepsy Muscle wasting- pendulous abdomen, sway back Fat redistribution- bulging supraorbital fat pads, cresty neck, fat accumulation around tailhead Infertility www.lazyhorserescue.org Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

8. Diagnostics: CBC/Chem/UA Dexamethasone Suppression Test (DST)-fail to suppress cortisol (> 1μg/dl) ACTH levels- >50pg/ml in horses and >27pg/ml in ponies supports diagnosis Baseline cortisol concentrations Urinary corticoid/creatinine ratio ACTH stimulation test Combined DST and ACTH stimulation test Thyrotropin-releasing hormone (TRH) stimulation test Combined DST and TRH stimulation test Resting insulin concentrations Glucose tolerance test Insulin tolerance test Imaging Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

9. Treatment: Dopamine agonists Pergolide and Bromocriptine Serotonin antagonist Cyproheptadine Supplements Magnesium & chromium picolinate Vitex (chasteberry extract, V. agnus-castus) *Treat all pathologic conditions (i.e., laminitis & infections) www.smartpakequine.com Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

10. Necropsy findings: Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

13. Pituitary Adenoma Histopathology : Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

14. Miller, M.A., I.D. Pardo, L.P. Jackson, G.E. Moore and J.E. Sojka. “Correlation of Pituitary Histomorphometry with Adrenocorticotrophic Hormone Response to Domperidone Administration in the Diagnosis of Equine Pituitary Pars Intermedia Dysfunction.” Veterinary Pathology 45 (2008): 26- 38. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

15. Ultrastructural Findings : Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

16. References : 1. Banks, William. Applied Veterinary Histology. Baltimore, MD: Williams & Wilkins, 1981. 2. Capen, Charles. “Tumors of the Endocrine Glands.” Tumors in Domestic Animals. Ed. Donald Meuten. Ames, IA: Iowa State Press, 2002. 616-620. 3. McFarlane, D., M.T.Donaldson, T.M. Saleh and A.E. Cribb. “The Role of Dopaminergic Neurodegeneration in Equine Pituitary Pars Intermedia Dysfunction (Equine Cushing’s Disease).” Proceedings of the 49th Annual Convention of the American Association of Equine Practitioners, New Orleans, Louisiana, USA, 21-25 November 2003. Internet Publisher: International Veterinary Information Service. 4. Miller, M.A., I.D. Pardo, L.P. Jackson, G.E. Moore and J.E. Sojka. “Correlation of Pituitary Histomorphometry with Adrenocorticotrophic Hormone Response to Domperidone Administration in the Diagnosis of Equine Pituitary Pars Intermedia Dysfunction.” Veterinary Pathology 45 (2008): 26- 38. 5. Toribio, Ramiro. “Pars Intermedia Dysfunction (Equine Cushing’s Disease).” Equine Internal Medicine. Ed. Liz Fathman. St. Louis, MN: Saunders, 2004. 1327-1338. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

17. BIG Thanks to: Pathologists: Drs. Tanya LeRoith & Geoff Saunders Equine Clinicians: Drs. Mark Crisman & Jamie Wearn Resident Mates: Drs. Natalie Durrett, Anya Hawthorn, & Ron Tyler Histology Laboratory Technicians: Barbara Wheeler, Jill Songer, & Luther Vest Morphology Laboratory Manager: Kathy Lowe Media: Jerry Baber Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

18. Go Pack!Go Hokies! QUESTIONS? Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only