A B Figure S1 pRS425pRS425-FCP1 Rpb3p ChIP efficiency (relative to wt promoter) C Ser2P / Rpb3p ChIP ratio (relative to wt promoter)

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A B Figure S1 pRS425pRS425-FCP1 Rpb3p ChIP efficiency (relative to wt promoter) C Ser2P / Rpb3p ChIP ratio (relative to wt promoter)

Ser2P ChIP efficiency (relative to wt promoter) RPB1rpb1-N488D Figure S2 A B C RPB1rpb1-N488D Ser2P / Rpb1p ChIP ratio (relative to wt promoter) Relative Rpb1p occupancy

Figure S3 ChIP Fcp1-myc (% Input) rat1-1 A B Ctk1-HA / Rpb3 ChIP ratio Ctk1-HAFcp1-myc Fcp1-myc / Rpb3 ChIP ratio

wt rat1-1 wt rat1-1 Ser2P Rpb1p (H5) Total Rpb1p (Y-80) pRS315 rat1-D235A Figure S4