Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. PCR.

Slides:

Advertisements

Similar presentations

Fig. 4. TGF-α is one of the major cytokines in M2 conditioned media

Advertisements

Volume 115, Issue 3, Pages (September 1998)

Fig. 4. Single total plasma 8-isoprostane levels in both controls and type 2 diabetic patients. Diabetes Induces p66shc Gene Expression in Human Peripheral.

Supplemental Figure 2. (A) AtplaIVA-1 and AtplaIVA-2 null transcription lines for AtPLAIVA mRNA. RNAs from the relevant wild type Col were isolated.

Suppression by an h Current of Spontaneous Na+ Action Potentials in Human Cone and Rod Photoreceptors Invest. Ophthalmol. Vis. Sci ;46(1):

Htr-9 stimulates ICAM-1 gene expression in human ASM cells.

Retroviral-Mediated Gene Transduction of c-kit Into Single Hematopoietic Progenitor Cells From Cord Blood Enhances Erythroid Colony Formation and Decreases.

by Jurgen Seppen, Simon C. Barry, Brandon Harder, and William R. A

Exposure to fluid shear stress modulates the ability of endothelial cells to recruit neutrophils in response to tumor necrosis factor-α: a basis for local.

Cytokine and chemokine expression of flucloxacillin-specific T cell clones (TCC) of patient P1. Cytokine and chemokine expression of flucloxacillin-specific.

Oligonucleotide sequences of polymerase chain reaction (PCR) primers and competitive templates. Oligonucleotide sequences of polymerase chain reaction.

Enhanced nasal cytokine production in human beings after in vivo challenge with diesel exhaust particles David Diaz-Sanchez, PhD, Albert Tsien, MD, Adrian.

Comparison of the banding patterns from randomly amplified polymorphic DNA PCR assays of sequential B pseudomallei isolates from each of the cases. Comparison.

Expression and regulation of MD-2s.

Lineage-Restricted Expression of Protein Kinase C Isoforms in Hematopoiesis by Alessandra Bassini, Giorgio Zauli, Giovanni Migliaccio, Anna Rita Migliaccio,

LP-BM5–induced increases of cytokine levels in the hind paw skin of B6 mice. LP-BM5–induced increases of cytokine levels in the hind paw skin of B6 mice.

Expression of IL-6, IL-8, and RANTES on human bronchial epithelial cells, NCI-H292, induced by influenza virus A Satoshi Matsukura, MD, Fumio Kokubu,

Figure 3 Transcripts of the splicing mutation (c

Supplementary Figure 1 FSC-A SSC-A HC UC CD

AGL15 Interacts in a Sequence-Specific Manner with a Noncanonical CArG Motif Present in the Regulatory Region of AtGA2ox6.(A) Enrichment of the AtGA2ox6.

Gemifloxacin inhibits cytokine secretion by lipopolysaccharide stimulated human monocytes at the post-transcriptional level F. Araujo, T. Slifer, S.

Comparison of cytokine profiles induced by ChiLob 7/4 and TLR ligands in ex vivo whole-blood stimulation assays. Comparison of cytokine profiles induced.

Volume 115, Issue 1, Pages (July 1998)

Structure of a typical back-propagated multilayered perceptron used in this study. Structure of a typical back-propagated multilayered perceptron used.

Volume 114, Issue 6, Pages (June 1998)

Volume 64, Issue 6, Pages (December 2003)

Determination of IL-5+ and IL-6+ cells in the lamina propria of the small intestines of mice receiving L. casei administration. Determination of IL-5+

PCR amplification of the ORF encoding the cytosolic p36 protein of M

Increased proinflammatory cytokine production is dependent on both the training interval and the resting time. Increased proinflammatory cytokine production.

Disruption of the svkA gene encoding severin kinase.

Prolactin gene expression in human ovarian follicular cells

Effects of TNF-α, IL-6, and IL-1β on 3T3-L1 adipocyte differentiation

Expression of VCAM-1 and inflammatory cytokines in injured carotid arteries in STZ-induced diabetic rats. Expression of VCAM-1 and inflammatory cytokines.

Contribution of individual serovars within serogroups isolated in the United States in Contribution of individual serovars within serogroups isolated.

Anti-inflammatory cytokine production is increased in trained monocytes. Anti-inflammatory cytokine production is increased in trained monocytes. (A) IL-10.

RT-PCR analysis of LTBP expression in human cell lines.

Expression of mRNA erythropoietin in the human retina.

Functional characterization of CD4+ T-cells with various antigen specificities after transduction with FoxP3. Functional characterization of CD4+ T-cells.

Trained immunity induction of glycolysis.

Trained immunity effects on cell morphology and numbers.

Cytokine changes in LPS-induced TNFα and spontaneous IL-6 production in whole-blood cultures. Cytokine changes in LPS-induced TNFα and spontaneous IL-6.

Changes in levels of transcripts encoding inflammatory cytokines and GFAP. The amounts of the PCR cDNA products were obtained from phosphorimager readings.

Immunization regimens that include a GLA-SE-formulated protein vaccine generate memory CD4 T cells. Immunization regimens that include a GLA-SE-formulated.

Summary of branch point mapping in P. falciparum.

Scattergrams showing IgG- and IgA-specific OD490 values measured with the Campylobacter-specific P18/P39 ELISA in sera from healthy controls and from GBS.

In vivo interaction of SAF-1 transcription factor with VEGF promoter in MDA-MB-231 cells. In vivo interaction of SAF-1 transcription factor with VEGF promoter.

MRNA expression IL-1α, IL-1β, IL-1Ra, IL-1RtI, ER α, ER β, and PR was analyzed by RT-PCR as described in “Materials and Methods.” Representative gel electrographies.

Dilution of anti-C6 monoclonal antibody shows the broader linear range of the Multiplex assay in comparison to standard ELISA. Shown is the dilution of.

Effect of anti-cyCD79b (10D10) and anti-cyCD79b (10D10)-MCC-DM1 on cytokine levels in cynomolgus monkeys. Effect of anti-cyCD79b (10D10) and anti-cyCD79b.

Reproducibility of CD4+ and CD8+ T-cell enumeration with cryopreserved PBMC after variable periods of storage in liquid nitrogen. Reproducibility of CD4+

Detection of PSA-mRNA by single (first panel, 710 bp) and nested (second panel, 455 bp) RT-PCR. Detection of PSA-mRNA by single (first panel, 710 bp) and.

T cell effector cytokines and staphylococcal products regulate CCL18.

Nested RT-PCR of Mammaglobin and CK19 mRNA in plasma and circulating cells of controls (C) and patients (T) with breast cancer. Nested RT-PCR of Mammaglobin.

Relation between the total white blood count and the absolute lymphocyte count prior to the first vaccination and the response to vaccination. Relation.

Identification of the recombinant structural proteins expressed in recombinant baculovirus-infected Sf9 cells by immunofluorescence assay with rabbit anti-FMDV.

RT-PCR Gel Blot Analyses of Cucurbita PP1 and PP2 mRNAs from Intergeneric Grafts of Cucumis sativus Scions on Cucurbita maxima or Cucurbita ficifolia Stocks.RT-PCR.

The increase in MHCI expression on tumor cells is dependent on cell-cell contact between NSCs and tumor cells. The increase in MHCI expression on tumor.

Group-averaged IL-10 levels (in pg/ml) from the Luminex assay for control, CFS with FM, and CFS without FM groups. Group-averaged IL-10 levels (in pg/ml)

Long-lived antibody-secreting cells in the bone marrow, induced by PfCelTOS vaccines containing various adjuvant formulations with emulsion doses of 0.02%

Long-lived antibody-secreting cells in the bone marrow, induced by PfCelTOS vaccines containing various adjuvant formulations with emulsion doses of 0.5%

Diisocyanate antigen–enhanced production of monocyte chemoattractant protein-1, IL- 8, and tumor necrosis factor-α by peripheral mononuclear cells of workers.

Time to culling for mastitis among J5 vaccinates and controls

Cytokines and cytokine receptors involved in type I immunity in tuberculosis. Cytokines and cytokine receptors involved in type I immunity in tuberculosis.

Antigen-specific cytokine production detected by multiplex bead assay (Luminex) 3 weeks after the 3rd immunization. Antigen-specific cytokine production.

Antigen-specific cytokine-producing cells (IFN-γ and IL-5) detected by ELISPOT assay 3 weeks after the 3rd immunization. Antigen-specific cytokine-producing.

Analysis of TNF-α gene transcription and mRNA accumulation in THP-1 cells. Analysis of TNF-α gene transcription and mRNA accumulation in THP-1 cells. (A)

GK1 enhances the secretion of proinflammatory cytokines resulting from cognate interactions between activated DCs and T cells. GK1 enhances the secretion.

Determination of CD-206 and TLR-2 receptors in immune cells in lamina propria of the small intestine of mice given L. casei. Determination of CD-206 and.

Time to culling for all reasons among J5 vaccinates and controls

Variability between laboratories in titer estimations (expressed as GCV on the y axis) tends to be higher for samples in low measurement ranges and to.

Presentation transcript:

Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. Cytokine expression induced by ligated-intestinal-loop assay using RT-PCR. PCR products were applied to 2% agarose gels. Eleven kinds of cytokines (IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, and IL-12p35 [lanes 1B to 12]; IFN-γ [IFN]; TNF-α [TNF]; TGF-β [TGF]; and MIP-2 [MIP]) expressed in PP cells (a) and LP cells (b) were detected by RT-PCR using specific primer pairs. As an internal control, a DNA fragment of β-actin (actin) was also amplified. The data represent at least three separate experiments. Akinobu Kajikawa et al. Clin. Vaccine Immunol. 2010; doi:10.1128/CVI.00337-09