1. Fig. 4. Single total plasma 8-isoprostane levels in both controls and type 2 diabetic patients.
Diabetes Induces p66shc Gene Expression in Human Peripheral Blood Mononuclear Cells: Relationship to Oxidative Stress
J Clin Endocrinol Metab. 2005;90(2): doi: /jc
J Clin Endocrinol Metab | Copyright © 2005 by The Endocrine Society

2. Fig. 5. Linear regression between total plasma 8-isoprostane levels and p66^shc gene expression in diabetics (r² = 0.47; P = ).
Diabetes Induces p66shc Gene Expression in Human Peripheral Blood Mononuclear Cells: Relationship to Oxidative Stress
J Clin Endocrinol Metab. 2005;90(2): doi: /jc
J Clin Endocrinol Metab | Copyright © 2005 by The Endocrine Society

3. Fig. 1. A representative experiment of three performed of p66^shc gene expression in monocytes and lymphocytes evaluated with primer set A (see Subjects and Methods). p66^shc RT-PCR products were detected in both monocytes (lane 1) and lymphocytes (lane 2) of healthy subjects. Lane 3, p66^shc negative with no cDNA control; lane 4, blank. Mwm, Molecular weight marker.
Diabetes Induces p66shc Gene Expression in Human Peripheral Blood Mononuclear Cells: Relationship to Oxidative Stress
J Clin Endocrinol Metab. 2005;90(2): doi: /jc
J Clin Endocrinol Metab | Copyright © 2005 by The Endocrine Society

4. Fig. 2. p66^shc gene expression in monocytes and lymphocytes evaluated with primer set B (see Subjects and Methods). p66^shc RT-PCR products were detected in both monocytes (lane 1) and lymphocytes (lane 2) of healthy subjects. Fibroblasts were used as a positive control (lane 3). A representative experiment of three performed is shown.
Diabetes Induces p66shc Gene Expression in Human Peripheral Blood Mononuclear Cells: Relationship to Oxidative Stress
J Clin Endocrinol Metab. 2005;90(2): doi: /jc
J Clin Endocrinol Metab | Copyright © 2005 by The Endocrine Society

5. Fig. 3. Densitometric analysis of p66^shc gene expression in PBM of type 2 diabetic patients and healthy controls. Gene expression of p66^shc was assessed by RT-PCR, using primer set B, after analysis of a kinetic curve set using an increasing number of cycles from 25–60, in order of 5, to determine the number of cycles corresponding to the exponential phase, as reported in Subjects and Methods. Values were adjusted for expression of the housekeeping gene β-actin and expressed as densitometric units (d.u.). *, P < Top, Representative polyacrylamide silver-stained gel of p66^shc PCR products from two healthy controls (lanes 1 and 2) and two type 2 diabetic patients (lanes 3 and 4).
Diabetes Induces p66shc Gene Expression in Human Peripheral Blood Mononuclear Cells: Relationship to Oxidative Stress
J Clin Endocrinol Metab. 2005;90(2): doi: /jc
J Clin Endocrinol Metab | Copyright © 2005 by The Endocrine Society