1. Comparison of cytokine profiles induced by ChiLob 7/4 and TLR ligands in ex vivo whole-blood stimulation assays.
Comparison of cytokine profiles induced by ChiLob 7/4 and TLR ligands in ex vivo whole-blood stimulation assays. LPS was the most potent ligand for stimulating release of cytokines. Soluble ChiLob 7/4 (CL7/4) and CpG were not effective at producing cytokines above unstimulated levels. The cytokine expression profile for cross-linked ChiLob 7/4 was similar to that produced by LPS, producing significantly high concentrations (*, P < 0.05) of MIP1α, MIP1β, TNFα, IL-6, IL-8, and IL-12 compared with unstimulated, although the levels of MIP1α, MIP1β, IL-6, IL-8, and IL-12 produced with cross-linked ChiLob 7/4 were significantly lower than that produced by LPS (**). Low but significant levels of IL-4 and IL-10 above unstimulated were also produced (***). The results show the mean response of 3 donors.
F. Chowdhury et al. Cancer Immunol Res 2014;2:
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