Automatization for cryopreservation SMARTFREEZER® Automatization for cryopreservation Daniele Pericolini, Marketing Manager Angelantoni Life Science

BIOLOGICAL SAMPLE STORAGE Samples are useful as long as they are stored reliably, protected from accidental and intentional physical damage. Samples are precious!! Months of hard work Unique patient sample A lot of money spent Many people involved

-196°C liquid or vapor phase TRADITIONAL STORAGE SYSTEMS ULT freezers, -80°C Liquid Nitrogen Tank -196°C liquid or vapor phase

LIMTS OF TRADITIONAL STORAGE SYSTEMS Manual management systems do not provide a fast and secure recovery of the samples. Where is my sample? Each user adopts his own criteria Many different systems coexist: handwriting, notes, excel files, ​​"in house" databases Samples stored without any order Lack of traceability of the individual sample

LIMTS OF TRADITIONAL STORAGE SYSTEMS A wrong labeled sample may be impossible to find Ice formation on the surface of bottles and boxes heavily complicates the search of samples Access to the samples by the operator is not recorded Lack of association between inventory data and physical location of samples The storage conditions (temperature, humidity) are not reliably monitored

LIMTS OF TRADITIONAL STORAGE SYSTEMS Sample retrieval exposes many other samples to dangerous warming events The exposure time of the samples at high temperatures is not traced The number of freeze / thaw cycles is not traced Sample mix-ups and losses

LIMTS OF TRADITIONAL STORAGE SYSTEMS Loss of data identification / location Deterioration of samples Lack of traceability of the cycles of freeze / thaw Human error Loss of a sample can result in priceless economic and scientific damage

SMARTFREEZER® The only completely automated device to store biological samples at cryogenic temperature in N2 vapors Fully automated system No exposure of the sample to unnecessary freezing-thawing cycles No operator exposure to liquid nitrogen Automatic tracking of the sample, historical data and inventory Integration with LIMS (Laboratory Information Management System)

SMARTFREEZER® Automation Electronic rack Storage Cooling system

HOW SMARTFREEZER® WORKS Vials are usually pre-frozen Then loaded into Smartfreezer drawer Identified Loaded

HOW SMARTFREEZER® WORKS Pneumatic gripper Withdrawal of a single sample (single picking/cherry picking) Individual identification of the sample by dot code or barcode

HOW SMARTFREEZER® WORKS Service Tools Camera installation Digital camera, HD

HOW SMARTFREEZER® WORKS Service Sw: User friendly touch Screen Interface Holes calibration Fine adjustment Loading/unloading simulation Vials identification system

Smart Freezer Application HOW SMARTFREEZER® WORKS Smart Freezer Application User friendly touch Screen Interface Full traceability of loading/unloading Only authorized people have access to the samples Interface with customer LIMS (Limfinity, Centraxx, etc..)

SMARTFREEZER® KEY POINTS A single sample at a time is retrieved: no exposure of other samples to dangerous warming events The right sample is available within few seconds Cross contamination is not possible Operator is not exposed to ultra low/cryogenic temperatures Samples are always identified and tracked No ice on samples Mix ups and losses are not possible User access to samples is tracked Prevention of accidental and intentional physical damage Built in match of data inventory and physical sample locations Complete history of storage operations and temperature conditions

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