Fig. 4 Identification of C

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Fig. 4 Identification of C Fig. 4 Identification of C. elegans proteins with tissue- and subcellular-specific localizations. Identification of C. elegans proteins with tissue- and subcellular-specific localizations. (A) The number of proteins identified above background from the tissue or subcellular location for each of the eight locations indicated. (B) The total number of proteins identified in our experiments that were detected in different locations or detected as being specific to a location. (C) The number of proteins we identified in the indicated tissue but not in the other three tissues. For each tissue, three categories of proteins are shown: those that are specific to the cytoplasm (orange), those that are specific to the nucleus (blue), and those that are not specific to either compartment (gray). (D) Comparison of the identified tissue-specific proteins to a data set of predicted mRNA expression (22). The data presented are the average of all the mRNA expression prediction scores for each tissue-specific protein in each of the four tissues. Higher prediction scores are more likely to be expressed in that tissue. Each column represents proteins identified as specific to that tissue, compared to the predicted mRNA expression of the tissue in each row. The highest average score in each column is shaded in green, and all other scores in the column are shaded in red. (E) Gene Ontology (GO) term enrichment analysis of proteins identified in our experiments as specific to either the cytoplasm or the nucleus. (F) Pie chart of the nucleus-specific proteins we identified in our experiment and whether they have a GO term location of either the nucleus or the cytoplasm, both, or neither. Aaron W. Reinke et al. Sci Adv 2017;3:e1602426 Copyright © 2017, The Authors