1. Proteins enriched in HCs are strongly associated with genetic hearing/balance impairment.
Proteins enriched in HCs are strongly associated with genetic hearing/balance impairment. A, Distribution of identified genes associated with IE impairment in transgenic mice reveals a disproportionately high number of genes in the HC-only subgroup compared with SE- or IE-only subgroups relative to the total number of genes found in each subgroup (see Fig. 3B). Fisher's exact test, p = B, Transcriptomic data from P4–P7 GFP+ HCs from Scheffer et al. (2015), where fold change Coch/Utr represents differential gene expression levels between cochlear and utricular HCs. Number of transcripts identified only in cochlear or utricular samples are indicated with green and purple labels, respectively. C, Mapping of HC-only proteomic-derived genes (see Fig. 3B) to mRNA rank, where higher rank indicates more highly expressed in cochlear compared with utricular HCs (from B). D, Distribution of identified genes orthologous to human deafness genes reveals a disproportionately high number of genes in the HC-only subgroup compared with SE- or IE-only subgroups relative to the total number of genes found in each subgroup (see Fig. 3B). Fisher's exact test, p = E, Protein identification summary across organ of Corti replicates. Filled black circles indicate rank-ordered abundance of proteins identified in the replicate with the largest number of total proteins identified. The number of replicates in which a given protein was identified is indicated by the color of the line connecting the circle to the abscissa (white, purple, or orange for one, two, or three replicates, respectively). Bar plot (inset) summarizes number of proteins identified in one, two, or three replicates. Mouse orthologs of human deafness genes identified in additional organ of Corti replicates, but not identified in samples in D, are indicated with gray circles. For D and E, genes for nonsyndromic deafness are shown in bold, nonsyndromic and syndromic in bold italic, and syndromic in plain text.
Ann E. Hickox et al. J. Neurosci. 2017;37:
©2017 by Society for Neuroscience