Scheme for recovery of viral nucleic acid from microarray spots.

Slides:



Advertisements
Similar presentations
D.B. Andreychuk1, V.V. Drygin1
Advertisements

Electron microscopy of C. albicans cell wall.
Inferring chromatin organization.
Increase in biologically effective UV irradiance (biologically effective dose) with decreasing simulated ozone column thickness as measured by the spore.
Frequency of inactivated spores of B
Shielding of bacterial spores against galactic cosmic radiation by meteorite material. Shielding of bacterial spores against galactic cosmic radiation.
Allosteric site of B. subtilis PRPP synthase.
Cross-section of a spore of B. subtilis.
Biochemical function of PRPP
Solar spectrum in space (thin line) and on Earth's surface (thick line). Solar spectrum in space (thin line) and on Earth's surface (thick line). Below.
Human ARDS. Photomicrographs from the lungs of 2 different patients with ARDS, stained with hematoxylin and eosin (H&E) are shown. Human ARDS. Photomicrographs.
Phylogenetic analysis of PurR from low-GC Gram-positive bacteria.
Figure S4 control-siRNA Day 0 fes A-siRNA Day 3 fer 1-siRNA
Phrap assemblies visualized with the Consed (53) program.
Catalytic strategies. Catalytic strategies. The coordination of Mg2+ (shown as blue spheres) of the active site to the substrates, to amino acid residues,
Possible secondary structure of the S. enterica prs leader.
Alignments of the complete genome sequences of the B
CTL killing and hepatocyte replacement.
The parental nondifferentiated ileocecal adenocarcinoma HCT-8 cell line and fully differentiated HCT8-MTX cells. The parental nondifferentiated ileocecal.
Structures of hydrophobic signaling molecules produced by diatoms and bacteria. Structures of hydrophobic signaling molecules produced by diatoms and bacteria.
Verigene solid-phase microarray.
(A) Scanning electron micrograph of bacteria on the surface of a corn (Z. mays) leaf. (A) Scanning electron micrograph of bacteria on the surface of a.
Summary of major events in Earth history relating to the appearance of eukaryotic groups and the appearance of O2 in the atmosphere and in marine environments.
Structures of P. aeruginosa ExoU and its chaperone, SpcU (PDB ID 3TU3), shown as a cartoon. Structures of P. aeruginosa ExoU and its chaperone, SpcU (PDB.
Electron micrograph of negatively stained protofilaments assembled in vitro from E. coli FtsZ (1 μM FtsZ, 50 mM MES [morpholineethanesulfonic acid] [pH.
Schematic representation of the hydrolysis of amorphous and microcrystalline cellulose by noncomplexed (A) and complexed (B) cellulase systems. Schematic.
Antibiofilm strategies arising from fundamental research.
Hector H. García et al. Clin. Microbiol. Rev. 2002; doi: /CMR
UV resistance of spores and vegetative cells of B
T4P gene clusters in C. perfringens and C. difficile.
Protein structures of various PFT classes.
Extreme resistance of D
Workflow summary of printed microarrays.
SMase cleavage sites in sphingomyelin and cellular activities of the reaction products. SMase cleavage sites in sphingomyelin and cellular activities of.
Physiological effects of heat shock.
Sources of energy and building blocks in oxidatively stressed D
Diagram of the relationship between the T4 transcriptional pattern and the different mechanisms of DNA replication and recombination. Diagram of the relationship.
Recovery template. Recovery template. The recovery template (internal control) has the same sequence as the PCR product except the probe region has been.
Prophage genome map of the C. diphtheriae prophage encoding DT
Schematics depicting a stylized cross section of dark ocean habitats (top; adapted from reference 259 by permission of Macmillan Publishers Ltd., copyright.
Roberta B. Carey et al. Clin. Microbiol. Rev. 2018; doi: /CMR
Molecular models for module exchange between phages.
Photographs of representative sediment habitats in the dark ocean.
MEED exposure facility mounted on the camera beam of the lunar orbiter of the Apollo 16 mission (A) and sample cuvette for exposing dry layers of microorganisms.
Binding of mRNA to the 30S ribosomal subunit.
Electron micrographs of bacteriophage T4.
The Scorpions primer is an extension of molecular beacons.
Concerted model for maltose transport.
Integration of the kDNA minicircle sequence into the genome of a rabbit with Chagas' disease. Integration of the kDNA minicircle sequence into the genome.
General scheme of the events triggered by C
Linear relationship between the dose of ionizing radiation and, from top to bottom, the extent of DNA degradation and the delay in DNA synthesis. Linear.
Conserved motifs in the ABC
Absorption spectra of PRs in relation to light attenuation patterns in different marine environments. Absorption spectra of PRs in relation to light attenuation.
The selective advantage (relative fitness) of a mutant genome (with its associated cloud) alters the rate of dominance in a population. The selective advantage.
Thermodynamic cycle for ligand binding to a periplasmic-BP.
(A and D) Modified Ziehl-Neelsen acid-fast staining of Cryptosporidium parvum/C. hominis oocysts (A) and a Cystoisospora belli oocyst (D). (A and D) Modified.
Intrastrand biases (nucleotide skew) in the T4 genome.
Characterization of CWR22 xenografts and XMRV-related sequences.
General overview of the bioinformatic pipelines for the 16S rRNA gene microbial profiling and shotgun metagenomics. General overview of the bioinformatic.
Mono-ADP-ribosylation cycle and the corresponding products of protein mono-ADP-ribosylation. Mono-ADP-ribosylation cycle and the corresponding products.
Surface expression of cell wall proteins.
Simplified representation of several types of genetic modifications that can alter the composition of viral quasispecies. Simplified representation of.
Repair kinetics of radiation-induced DNA damage under microgravity conditions. Repair kinetics of radiation-induced DNA damage under microgravity conditions.
Sequence alignment of colicin lysis proteins.
Timeline of 16S rRNA genes represented in the database.
Autoradiography of [3H]DAP incorporation (a and b) and of [3H]leucine incorporation (c). Autoradiography of [3H]DAP incorporation (a and b) and of [3H]leucine.
(A) Crystal structure of the monomer of PGI/PMI from Pyb
Acquisition of global regulators during the evolution of streptomycetes. Acquisition of global regulators during the evolution of streptomycetes. The multilocus.
Structure of an ATP-bound ABC dimer.
Actin tails formed by Rickettsia spp.
Presentation transcript:

Scheme for recovery of viral nucleic acid from microarray spots. Scheme for recovery of viral nucleic acid from microarray spots. Hybridized viral sequences were physically scraped from a DNA microarray spot using a tungsten wire probe mounted on a micromanipulator, while the spots were visualized under fluorescence microscopy. Subsequently, the virus was identified by nucleic acid amplification, cloning, and sequencing. (Reprinted from reference 512 with permission.)‏ Cécile Voisset et al. Microbiol. Mol. Biol. Rev. 2008; doi:10.1128/MMBR.00033-07