1. Catalytic strategies.
Catalytic strategies. The coordination of Mg2+ (shown as blue spheres) of the active site to the substrates, to amino acid residues, and to water molecules (shown as red spheres) is schematically illustrated by blue lines. (A) cAMP-dependent protein kinase A (PDB code 4hpu) (201). (B) PRPP synthase. R5P, ribose 5-phosphate. MgA was designated MG2, and MgB was designated MG1 before (54). (C) Hexose 1-phosphate nucleotidyltransfrease, based on the structure of N-acetylglucosamine 1-phosphate uridylyltransferase (PDB code 4g87). MgB coordinates to aspartate and asparagine residues (192). (D) DNA polymerase based on the structure of DNA polymerase β (PDB code 1bpy) (176). The three acidic residues are usually aspartates, but occasionally one aspartate is replaced by a glutamate (185, 186). The structure is valid also for RNA polymerases (187).
Bjarne Hove-Jensen et al. Microbiol. Mol. Biol. Rev. 2017; doi: /MMBR