Summer Assignment: Virtual Lab Seed Experiment 1. Research Notebook Set-Up (a tutorial for you!) 2. Data Collection 3. Completing Virtual Seed Lab in the Notebook

Lab Notebook Set-Up Objective: Students will: Set-Up their Research Notebook (Part 2 of Summer Assignment). Collect Data on Seed Growth based a specified Independent Variable (Part 3 of Summer Assignment). Display Data collected in Data Tables and Graphs (Part 3 of Summer Assignment as well).

Research Lab Notebook - lets get started: Here is an Overview of how we will set up our Notebooks for every lab: Page 1: Title Page Page 2 -4: Table of Contents Page 5 leave blank (just in case) Page 6 - 13: Label with: Page number Date Title of Lab/experiment OR Research Question? Did you remember to add the date, title and page number to your Table of Contents? If not, do so!

Only write on the Right Page: Example: Research Biology 2018-2019 Your Name (Jane/John Doe Riverside High School, Leesburg, VA 20176 This line represents the spiral

Time to Set Up your Biology Research Notebook! ***We never write on the left page of our Notebook!!!! Title Page: open to your first page (should be the right page) See next slide: the information needs to be written in the very center of the page! In the top right hand corner write the date. In the bottom right hand corner write the “1” (its your page number!).

Your Name (Jane/John Doe Set up your Title Page: (left page) (leave blank) . (right page) Date Example: Research Biology 2018-2019 Your Name (Jane/John Doe Riverside High School, Leesburg, VA 20176 1.

Time to Set Up your Biology Research Notebook! ***We never write on the left page of our Notebook!!!! Table of Contents: Turn to your next page (right side only!) At the top, in the center, write “Table of Contents” either in ALL CAPS or underlined. In the top right hand corner write the date. In the bottom right hand corner write the “2” (its your page number!).

Does your table of contents look like this? (left page) (Leave blank) . (right page) Date Table of Contents Date Title Page # ___/___/18 Virtual Seed Lab 6-13 2. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Does your table of contents look like this? (left page) (Leave blank) . (right page) Date Table of Contents Date Title Page # 3. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Does your table of contents look like this? (left page) (Leave blank) . (right page) Date Table of Contents Date Title Page # 4. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!) Leave this page blank! (left page) (Leave blank) . (right page) 5. Set up 3 pages (2 - 4) for the title pages (leave page 5 blank!)

Notebook Set-Up Cont. Virtual Seed Lab: Research Question & Model Turn to your next page (right side only!) At the top, in the center, write “Virtual Seed Lab” either in ALL CAPS or underlined. In the top right hand corner write the date. In the bottom right hand corner write the “6”. Below the Title, drop down 2-3 lines to write the headers for this page (always start to the left). First Header: Research Question: Second Header: Research Model: (leave a few spaces between headers) See next slide and complete this page.

Lets add our Model to the (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 6. Research Question : What do seedlings need to germinate? Research Model : Germinating Seeds water CO2 light

Notebook Set-Up Cont. Virtual Seed Lab: Student Understandings Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “7”. Below the Title, drop down a few spaces and write the Header, “Student Understandings” Repeat for Page 8. See next slides to complete these page. We will fill in this section in class This is where we document background information about the organism(s) and processes we are researching (more to come on this in class!)

Add more pages to this experiment: (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 7. STUDENT UNDERSTANDINGS:

Add more pages to this experiment: (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 8. STUDENT UNDERSTANDINGS:

Notebook Set-Up Cont. Virtual Seed Lab: Experimental Design Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “9”. Below the Title, drop down a few spaces and write the Header, “Experimental Design” See next slide to set this page.

Add more pages to this experiment: (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 9. EXPERIMENTAL DESIGN: will complete the parts with red together in class! Research Question: Null (Ho) Hypothesis: Alternative (Ha) hypothesis: leave a 3-4 lines, we will add later during class Independent Variable: Experimental Group(s): will fill in based on last name (a chart is available in the next slides) Control: Seeds Soaked Overnight (10 hrs light and 14 hrs Dark at 23oC) Dependent Variable: see next slide Constants: we will add later during class

Filling out the Experimental Design: Research Question: What do seedlings need to germinate? Hypothesis Null: The Independent Variable (IV) will not effect the Dependent Variable (DV). You need to use your IV and DV here! What is your Independent Variable (IV) for the Virtual Seed Lab? This is going to vary and be based on your last name. See next slide. What is the Dependent Variable (DV)? Measurement of root and shoot length (cm)

Filling out the Experimental Design: What is your Independent Variable (IV) for the Virtual Seed Lab? This is going to be based on your last name. Instructions for these will come in next slides. Last Name Independent Variable Control vs Experiment Group A-E Time (exposed to light) A1 10 hrs light, 14 hrs dark vs. B1 24 hrs dark F-K A1 10 hrs light, 14 hrs dark vs. C1 24 hrs light L-O Type of Light A1 10 hrs light, 14 hrs dark vs. D1 Daylight P-T Rotation of seeds A1 soaked, not rotated vs. A2 soaked & rotated U-Z Presence of water A1 soaked vs. A3 not soaked

Notebook Set-Up Cont. Virtual Seed Lab: Materials & Procedures Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “10”. Below the Title, drop down a few spaces and write the Header, “Materials”. Leave 10 lines and write the Header “Procedures”. MATERIALS SHOULD BE BULLETED PROCEDURES SHOULD BE NUMBERED See next slide for this page.

Add more pages to this experiment: (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 10. MATERIALS: (bullet list) PROCEDURES: (numbered steps)

List of Materials to write into Notebook: Seed packets (this experiment uses Peas, Little Marvel) Re-sealable plastic bags (sandwich or quart size) Paper towels or napkins Water Tape Cardboard (optional) Paper clips (optional) Binder clips (optional) Scissors

Procedures to Write into Notebook: Day 1, soak the seeds (this experiment uses Peas, Little Marvel) overnight in water. Day 2, Place a wet paper towel in a ziplock bag and place 5-10 seeds on the paper towel in the bag. Blow air into the bag and seal. On Day 3-4, observe the growth of the seeds.

Notebook Set-Up Cont. Virtual Seed Lab: Data Tables Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “11”. Below the Title, drop down a few spaces and write the Header, “Data Table”. See next slide for this page.

Add more pages to this experiment: (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 11. DATA TABLES: refer to #8 in part 3 for pasting provided data tables on this page

Notebook Set-Up Cont. Virtual Seed Lab: Graphs Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “12”. Below the Title, drop down a few spaces and write the Header, “Graph”. See next slide for this page.

Add more pages to this experiment: (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 12. GRAPHS: refer to #9 in Part 3 for completing the two required graphs 2 Graphs! 1 for ROOTS and 1 for SHOOTS Be sure to include the following for your Graph: Title (The Effect of the IV on the DV) Dependent variable on Y axis Independent variable on X axis Axis labeled Scales drawn on axis Key included Drawn neatly (with a ruler – no free Hand drawing of your graph)

Notebook Set-Up Cont. Virtual Seed Lab: Results Paragraph Turn to your next page (right side only!) At the top, write the title and date again. In the bottom right hand corner write “13”. Below the Title, drop down a few spaces and write the Header, “Results”. See next slides for guidance on this page.

Add more pages to this experiment: (left page) (right page) Date VIRTUAL LAB SEED EXPERIMENT 13. Results: (in paragraph form) Will do this in class! Just set up this page! State your Research Question. Claim: Make a claim - (statement that answers the research question) Evidence: Provide evidence from the data that was collected Reasoning - describe how the science principles explain the results

Analysis of Data/Results This section will be done in class! This is just a brief overview of what will go in a Results section! Making a Claim from the Data for the Control seeds and the experimental seeds on days 1-10: Look at your graphs. Do you notice any trends in the data? Which days do the seeds start to grow a root, or a shoot? Do roots and shoots grow at the same rate? Does the data help us to answer our research questions: How do seedlings develop over time?

*** be sure to go back and fill in the information on your Table of Contents!!!*** Move on to Part 3: Collect your Data All Done with Part 2!!! This direction sheet can be found in the Summer Assignment Packet