Volume 61, Issue 1, Pages (January 2002)

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Volume 61, Issue 1, Pages (January 2002)

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Volume 61, Issue 1, Pages 336-341 (January 2002) Quantifying porphobilinogen deaminase mRNA in microdissected nephron segments by a modified RT-PCR Dong Sun, George Seki, Shu Uwatoko, Akihide Nakao, Atsuo Goto, Toshiro Fujita, Satoshi Kimura, Shigeo Taniguchi, M.D. Kidney International Volume 61, Issue 1, Pages 336-341 (January 2002) DOI: 10.1046/j.1523-1755.2002.00105.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 Results of mutagenic reverse transcription-polymerase chain reaction (MRT-PCR) of porphobilinogen deaminase (PBGD) mRNA. The results of MRT-PCR of PBGD in four different nephron segments, glomeruli (GL), proximal convoluted tubule (PCT), cortical thick ascending limb of Henle (cTAL), and distal convoluted tubule (DCT) are shown. For each segment, RT (-) control is shown in the lane next to that of RT (+) sample. For each lane, the upper bands represent the products of genomic DNA and the lower bands that are lacking in RT (-) controls represent those of cDNA. Kidney International 2002 61, 336-341DOI: (10.1046/j.1523-1755.2002.00105.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 Results of MRT-PCR for PBGD and β-actin. (A) The electrophoretic result of MRT-PCR products for PBGD and β-actin in glomeruli, and (C) that in PCT are shown. In each panel, two left-sided lanes are for PBGD, and other 8 lanes are for β-actin. For β-actin, zero to 6 cycles of selective amplification of genomic DNA (SAG) are shown on the right-sided lanes. (B and D) Logarithmic values of CG ratios plotted for the SAG cycle numbers. Kidney International 2002 61, 336-341DOI: (10.1046/j.1523-1755.2002.00105.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 3 PBGD enzyme activity and mRNA quantities in the kidney and the liver. PBGD enzyme activity in the kidney inner medulla (IM), outer medulla (OM), cortex, and the liver homogenates are plotted for PBGD mRNA quantities measured by MRT-PCR. The two parameters show excellent correlation. Kidney International 2002 61, 336-341DOI: (10.1046/j.1523-1755.2002.00105.x) Copyright © 2002 International Society of Nephrology Terms and Conditions