1. Immunolocalization of phospholipase C isoforms in rat kidney
Janice P. Lea, Dilek Ertoy, Jennifer L. Hollis, Mario B. Marrero, Jeff M. Sands 
Kidney International 
Volume 54, Issue 5, Pages (November 1998)
DOI: /j x
Copyright © 1998 International Society of Nephrology Terms and Conditions

2. Figure 1
Expression of phospholipase C (PLC)-β1 in microdissected rat proximal convoluted tubule (PCT) and glomeruli. Western blot analysis of protein from rat PCT and glomeruli (100 μg protein loaded) identifies a 150 kD protein when probed with an affinity-purified polyclonal rabbit anti-rat antibody to PLC-β1. In CCDs and mTALs (20 μg protein loaded), protein was also detected with the PLC-β1 monoclonal antibody (results not shown).
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

3. Figure 2
Phospholipase C (PLC)-β1 in rat kidney.A, B, C and E represent the cortex, and D represents the inner stripe of outer medulla. Strong proximal tubule brush border (p) positivity is seen in panels A and B. Glomerular epithelial cells and capillary loops (panel C), and distal convoluted tubule (d) epithelial cell cytoplasms (panels A and C) reveal weak (1+), whereas mTALs (m) show moderate (2+) immunoreactivity (panel D). Collecting tubules (c) as seen in a medullary ray in (panel A) and inner stripe of outer medulla in panel D exhibit intense expression. Arterioles (a) are negative (panel A) in contrast to widespread vasa recta (v) positivity in panel D. Panel E is negative control where primary antibody was substituted with PBS (immunohistochemistry, 1:250 rabbit anti-rat polyclonal PLC-β1 antibody, A ×200, B ×400, C ×600, D ×400; E, Immunohistochemistry, negative control, ×200). Publication of this figure in color was made possible by a grant from Hoechst Marion Roussel, Inc.
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

4. Figure 3
Expression of phospholipase C (PLC)-γ1 in microdissected rat proximal convoluted tubule (PCT), cortical collecting duct (CCD), medullary thick ascending limb (mTAL), and glomeruli. (A) Western analysis of protein from microdissected rat PCT and of sieved glomeruli (100 μg protein loaded) identifies a 145 kD protein when probed with a monoclonal mouse antibody raised against bovine PLC- γ1. (B) Protein (20 μg protein loaded) from microdissected PCT, CCD, and mTAL also identify a 145 kD protein. Rat liver and brain were used as positive controls.
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

5. Figure 4
Phospholipase C (PLC)-γ1 in a rat kidney artery. Strong positivity of internal elastic lamina is shown (immunoreactivity, 1:100 rabbit anti-rat polyclonal PLC-γ1 antibody; ×400). Publication of this figure in color was made possible by a grant from Hoechst Marion Roussel, Inc.
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

6. Figure 5
Expression of phospholipase C (PLC)-δ1 in rat proximal convoluted tubule (PCT), cortical collecting duct (CCD), medullary thick ascending limb (mTAL), and glomeruli. (A) Western blot analysis of protein from rat glomeruli (30 μg protein) identifies an 85 kD protein when probed with a monoclonal antibody raised against bovine PLC-δ1. (B) Protein (20 μg) from rat PCT, CCD, or mTAL was detected when probed with the PLC-δ1 monoclonal antibody. Rat brain and liver were used as a positive control.
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

7. Figure 6
PLC-δ1 in outer stripe of outer medulla (A) and in inner medulla (B). (A) Thick ascending limbs (m) show intense staining. Descending thick loops of Henle (S3) are also positive but in less intensity and with negative brush-border. (B) Positivity of collecting ducts of papilla, accentuated on basolateral border of the cells (immunohistochemistry, 1:100 mouse anti-bovine monoclonal PLC-δ1 antibody; A ×400, B ×400). Publication of this figure in color was made possible by a grant from Hoechst Marion Roussel, Inc.
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions

8. Kidney International 1998 54, 1484-1490DOI: (10. 1046/j. 1523-1755
Kidney International  , DOI: ( /j x)
Copyright © 1998 International Society of Nephrology Terms and Conditions