Reduced Rab11 abundance impairs LFA-1 recycling and consequently LFA-1–dependent migration in T lymphocytes. Reduced Rab11 abundance impairs LFA-1 recycling.

Slides:

Advertisements

Similar presentations

MP cells are generated from naïve cells in the periphery.

Advertisements

FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.

Three different types of transfer functions with a codomain of [0,1].

Neurotensin receptor–1 and –3 complex modulates the cellular signaling of neurotensin in the HT29 cell line Stéphane Martin, Valérie Navarro, Jean Pierre.

Self-sensing of actuator position.

Endogenously presented LNP is recognized by KIR2DS2 in the context of HCV. Endogenously presented LNP is recognized by KIR2DS2 in the context of HCV. (A)

Regulatory CD4+ T cell–derived IL-10 is important for B cell differentiation and the GC response. Regulatory CD4+ T cell–derived IL-10 is important for.

Comparative analysis of the nuclear F-actin networks in A3

LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes, and reducing RhoB abundance impairs the.

Human PBMC-derived MERS-CoV–specific T cells are multifunctional.

Virus-specific T cell responses are detected in all MERS survivors.

Protection from autoimmunity is not due to the expansion of Treg subsets. Protection from autoimmunity is not due to the expansion of Treg subsets. (A.

ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. (A) Western.

LNP activates KIR2DS2+, but not KIR2DL2+, NK cells.

Enhanced expression of TLR7 protein in PBMCs from women.

Genetic FIP200 deletion impairs autophagy induction and causes T cell apoptosis. Genetic FIP200 deletion impairs autophagy induction and causes T cell.

Tukey boxplots overlaid on data points from objective and subjective measures, displaying results from study 1. Tukey boxplots overlaid on data points.

Tfr cells robustly secrete IL-10 after acute viral infection.

Tfr cell–derived IL-10 is important for B cell differentiation and the GC response. Tfr cell–derived IL-10 is important for B cell differentiation and.

Murine gingival MSCs and skin MSCs produce and secrete IL-1RA–EV

Blood Tfr cells are indicators of ongoing humoral activity.

AMPK directly increases mTORC2 catalytic activity.

FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.

Fig. 4 Labeling of Msmeg with DMN-Tre is fast and specific and depends on Ag85A function. Labeling of Msmeg with DMN-Tre is fast and specific and depends.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

Tumor control by necroptotic cells requires BATF3+cDC1 and CD8+leukocytes. Tumor control by necroptotic cells requires BATF3+cDC1 and CD8+leukocytes. (A)

Microrobots with different cell-carrying capacities under different grid lengths (lg) and burr lengths (lb). Microrobots with different cell-carrying capacities.

Self-sensing of actuator position.

Fig. 4 High P-eIF2α expression in human prostate tumors with loss of PTEN function is associated with increased risk of metastasis or death after surgery.

Slc7a5 expression in preosteoclasts is reduced in ovariectomized mice.

Autophagy protein abundance, but not autophagy flux, is altered in Cnot3-depleted heart. Autophagy protein abundance, but not autophagy flux, is altered.

MYC degradation screen identifies a compound that stabilizes MYC protein. MYC degradation screen identifies a compound that stabilizes MYC protein. (A)

Fig. 3 Biological function of TG2 and the interaction with MT-2.

Fig. 6 Metarrestin treatment reduces pre-RNA synthesis and Pol I occupancy at rDNA without changing rDNA chromatin states. Metarrestin treatment reduces.

GOLPH3 interacts with COG complex proteins.

Overview of DS-A*02:01 production and usage for affinity measurements.

Fig. 5 GP38 localization to the viral envelope and plasma membrane.

BMS blocks functional responses in primary immune cells driven by IFNα

Fig. 6 SNP rs is a functional SNP in 22Rv1 cells.

Fig. 4 HRI regulates BCL11A levels.

Fig. 2 HRI depletion elevates γ-globin in HUDEP2 cells.

Fig. 4 Cdc42 enhances the cellular uptake of EVs.

Fig. 5 Simultaneous absence of caspase-3 and -7 is required for significant decrease of caspase-8 and -9 activation in intrinsic apoptosis. Simultaneous.

AEGIS autonomous targeting process.

DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation. DGKζ and Cbl-b deficient T Cells have enhanced ERK1/2 and IκBα phosphorylation.

Western blot analysis of skin tissue from CJD and non-CJD patients

Fig. 4 PD-L1 expression is found in the spleen and the BM of mice transplanted with JAK2V617F-transduced bone marrow. PD-L1 expression is found in the.

Fig. 2 Regulation of CD73 and CD39 cell membrane expressions and extracellular adenosine levels by ERs in osteoprogenitor cells. Regulation of CD73 and.

Fig. 3 Regulation of CD73 and CD39 cell membrane expression and extracellular adenosine levels by ERs in osteoclasts. Regulation of CD73 and CD39 cell.

CD25 expression predicts effector and memory differentiation.

Fig. 2 ObR is cleaved and has reduced signaling capabilities after incubation with Mmp-2. ObR is cleaved and has reduced signaling capabilities after incubation.

Ponatinib (AP24534) inhibits MEKK3-KLF signaling and prevents formation and progression of cerebral cavernous malformations by Jaesung P. Choi, Rui Wang,

GSDMD localizes to the plasma membrane and is processed during NET formation. GSDMD localizes to the plasma membrane and is processed during NET formation.

by Geoffrey A. Smith, Jack Taunton, and Arthur Weiss

UNC drives MYC protein loss.

CAR expression promotes tonic activation of signaling networks.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Fig. 2 Increasing KLF17, CDH1, and LASS2 expression reduced malignant progression and promoted apoptosis of tumor cells. Increasing KLF17, CDH1, and LASS2.

NE cleaves and activates GSDMD.

Breakdown of incorrect participant responses.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Acute circadian disruption alters ILC3 cytokine secretion.

REV-ERBα deficiency reduces frequency and number of NKp46+ ILC3s.

Circadian gene expression in ILC3s is associated with rhythmic cytokine expression. Circadian gene expression in ILC3s is associated with rhythmic cytokine.

γδ T cells producing IL-17 are required for short-term memory.

Meningeal γδ T cells are biased toward IL-17 production.

Fig. 1 Generation and characterization of MeV-based vaccine candidates for Lassa virus. Generation and characterization of MeV-based vaccine candidates.

Fetal-derived γδ T cells infiltrate the meninges from birth.

Fig. 3 Three small molecules inhibit the transport of CCR5 and do not affect transport of CCR1 or CXCR4. Three small molecules inhibit the transport of.

Fig. 1 Differential anterograde transport of CCR5 and TNF.

Presentation transcript:

Reduced Rab11 abundance impairs LFA-1 recycling and consequently LFA-1–dependent migration in T lymphocytes. Reduced Rab11 abundance impairs LFA-1 recycling and consequently LFA-1–dependent migration in T lymphocytes. (A) Representative Western blots of T lymphocytes transfected with scrambled control or Rab11-specific siRNA were incubated with antibodies against total Rab11 and actin (mean knockdown efficiency, 88.7 ± 3.9%; means ± SEM; n = 3 experiments). (B) T lymphocytes transfected with the indicated siRNAs were incubated on immobilized ICAM-1 before random migration was observed by time-lapse microscopy. Individual cells were tracked and plotted with a common origin. A representative experiment of the pattern tracked by a single cell from 40 cells tracked is shown; n = 2 experiments. (C) Quantification of mean cell migration speed from the cells in experiments described in (B). Means ± SEM. (D) HSB-2 cells transfected with the indicated siRNAs and with biotinylated cell surface proteins were incubated on immobilized ICAM-1. The remaining biotin on surface-located cell membrane receptors was removed with reducing glutathione, and the internalized biotinylated LFA-1 was analyzed by Western blotting analysis of LFA-1 immunoprecipitates with streptavidin-HRP (first and second lanes). Reexposure of biotinylated LFA-1 to the surface was analyzed by reincubating T lymphocytes on immobilized ICAM-1 after the removal of biotinylated membrane receptors. Reexposed biotinylated membrane receptors were then removed with reducing glutathione, and the remaining intracellular biotinylated LFA-1 was subsequently analyzed by Western blotting analysis of LFA-1 immunoprecipitates with streptavidin-HRP (third and fourth lanes). (E) Mean band intensity from experiments similar to that represented in (D) (first and second lanes). Data are pooled and normalized to control. Means ± SEM; n = 3 experiments. (F) Mean band intensity from experiments similar to that represented in (D) (third and fourth lanes). Data are pooled and normalized to HRP intensity after internalization for siCTRL and siRhoB, as shown in (E). Means ± SEM; n = 3 experiments. (G) Representative Western blots from the analysis of isolated cytoplasmic (Cyt) or membrane (Mem) fractions from HSB-2 cells transfected with the indicated siRNAs. Blots were incubated with antibodies against total RhoB and LFA-1. (H) Quantification of the ratio between the mean band intensities of the cytoplasmic and membrane fractions from experiments similar to that shown in (G). Means ± SEM; n = 3 experiments. (I) Representative Western blots from isolated cytoplasmic or membrane fractions of HSB-2 cells transfected with scrambled control or RhoB siRNA and analyzed for total Rab11 and LFA-1. (J) Quantification of the mean band intensity ratio between the cytoplasmic fraction and the membrane fraction in (I). Left, Rab11; right, LFA-1. Pooled data from three independent experiments are shown. Means ± SEM. (H and J) *P < 0.05, **P < 0.01, ***P < 0.001, unpaired (two-tailed) t test. Malin Samuelsson et al., Sci. Signal. 2017;10:eaai8629 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works