Insulin promoter confers β-cell–specific GFP expression in pancreas.

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Insulin promoter confers β-cell–specific GFP expression in pancreas. Insulin promoter confers β-cell–specific GFP expression in pancreas. The dsAAV8-mIP-GFP vector was delivered by three different routes and analyzed in situ in the pancreas for β-cell–specific GFP expression. A: Two weeks after intraperitoneal delivery at a vector dose of 1 × 1012 viral genome particles/mouse. B: Two weeks and 4 months after intraductal delivery at a vector dose of 1.5 × 1011 viral genome particles/mouse. C: Two weeks after intravenous delivery with liver blockade at a vector dose of 1 × 1012 viral genome particles/mouse. In gross photos of the whole pancreas, notice the widespread and specific GFP expression in the islets after vector delivery by all three routes. Also notice strong GFP expression in β-cells of individual islet but not in the surrounding acinar cells. It is further confirmed by double immunofluorescent (IF) staining with anti-GFP (red) and anti-insulin (green) antibodies and counterstaining of all cell nuclei with DAPI (blue). Arrows highlight the islets. Scale bar: 100 μm. Zhong Wang et al. Diabetes 2006;55:875-884 ©2006 by American Diabetes Association