Biotechnology Practice Test

Question #1 How much material will a P-20 dispense if it reads 123?

Question #2 To join a piece of DNA from one source into another, which of these processes is used? a. Cloning b. Ligation c. Transformation d. Restriction digest e. DNA fingerprinting

Question #3 Which of these processes is used to cut up pieces of DNA? Gene therapy b. Ligation c. Transformation d. Restriction digest e. DNA fingerprinting

Question #4 What is made by the work of restriction enzymes?

Question #5 Which of the following is NOT a way that bacteria obtain new combinations of genes? Two bacteria exchange genetic info during conjugation Bacteria produce sex cells that undergo fertilization Viruses carry bacterial DNA from one bacteria to another Bacteria take up free pieces of DNA from their environment

Question #6 DNA is loaded into a gel. What is the charge, if any, of the DNA? Negative Positive Neutral It depends on base pair length

Question #7 Which of these is in the correct order for making a protein from a recombinant? Restriction digest, insertion, amplification, transformation Restriction digest, ligation, transformation, amplification Restriction digest, ligation, insertion, amplification Transformation, restriction digest, ligation, amplification

Question #8 Which of the following would be considered a transgenic organism? A plant grown in a lab from a single plant cell b. A sheep developed from an egg cell that was fertilized in the lab c. A sheep that produces a cow protein d. A sheep developed from an egg cell that was fertilized in the lab

Question #9 The process that involves inserting correct forms of a gene into a vector, and putting them back into the body is known as _______.

Question #10 Why do both sources of DNA in a recombinant have to be cut with the same restriction enzyme?