Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

Slides:

Advertisements

Similar presentations

Figure 4 K19del mutation alters CHP1 association into functional complexes, impairs NHE1 membrane targeting, and potentially compromises protein conformational.

Advertisements

Cdc42.GTP directly activates PAK4.

Implications of somatic mutations in the AML1 gene in radiation-associated and therapy-related myelodysplastic syndrome/acute myeloid leukemia by Hironori.

Patient-derived C-terminal mutation of FANCI causes protein mislocalization and reveals putative EDGE motif function in DNA repair by Luca Colnaghi, Mathew.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

Phosphorylation of serine 73 targets SREBP-1c for ubiquitination and proteasomal degradation Phosphorylation of serine 73 targets SREBP-1c for ubiquitination.

Volume 20, Issue 5, Pages (May 2011)

Mitophagy is independent of PINK1 and Parkin.

Pirh2 promotes p73 ubiquitination in vivo.

Suppl.Figure 1 Transfected HEK293 cells -130kDa -100KDa Blot: -V5

Pioglitazone inhibits aromatase expression in human preadipocytes.

HUWE1 regulates ubiquitination of Shoc2 and RAF-1.

Insulin regulates the expression and intra-membrane proteolysis of the recombinant HA-pSREBP-1c-Flag protein in McA-RH7777 cells Insulin regulates the.

APCFZR1 promotes BRAF ubiquitination in a D-box–dependent manner.

Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.

Figure 2 Luciferase assays of transiently transfected HEK 293 cells with reporter constructs containing the 766-bp wild-type KCNJ18 or c.-542 T/A mutant.

PKA phosphorylates Thr63 and Ser692 to increase HIF-1α stability.

Noah C. Jenkins, Jae Jung, Tong Liu, Megan Wilde, Sheri L

Figure 3 Mutation carrier–derived lymphoblastoid cell lines (LCLs) show decreased aconitase 2 activity and mitochondrial respiration deficiency compared.

Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.

Figure 2 Increased mitochondrial sensitivity to glucose reduction in lymphoblastoid cells from patients with primary lateral sclerosis Increased mitochondrial.

Figure 2 The K19del mutation affects the expression and solubility of CHP1 The K19del mutation affects the expression and solubility of CHP1 (A) Western.

Loss of FZR-1 results in elevated SAS-5 levels.

Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active.

Supplementary Figure 1 A B C SW620 HT29 SW620

Figure 3 Expression of mutant STIM1 in cultured cells and its effect on store-operated Ca2+ influx Expression of mutant STIM1 in cultured cells and its.

Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/-

PINCH-1 forms a complex with integrin-linked kinase (ILK) at focal adhesion sites. PINCH-1 forms a complex with integrin-linked kinase (ILK) at focal adhesion.

ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. (A) Western.

Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7 cells or RPE cells. Expression of the mutants of HP1α and the mutant of SYCE2 in MCF7.

HIPK2 decreases the stability of Notch1-IC through proteasome-dependent degradation. HIPK2 decreases the stability of Notch1-IC through proteasome-dependent.

TRAF4 is required for EGFR activation in response to EGF stimulation.

Figure 2 SORL1 mutants' overexpression increases β-amyloid secretion

Figure 2 DNM1 mutations inhibit transferrin uptake Inhibition of transferrin internalization in mammalian cell lines. DNM1 mutations inhibit transferrin.

Figure 5 Electron microscopy of transfected HeLa cells and Dnm1Ftfl neurons (A) Electron microscopy (EM) of HeLa cells transfected with wild type (WT)

RIF1 interacts with protein phosphatase 1 isoforms

Figure 3 ADAM22 mutant proteins do not bind to LGI1

Fig. 4. Clinically defined SMN mutants show alterations in the association with telomerase complex proteins. Clinically defined SMN mutants show alterations.

Fig. 5. Expression of either dFMRP or human FMRP does not induce eIF2α phosphorylation.(A) Analysis of eIF2α phosphorylation upon dFMRP expression. Expression.

Fig. 2. Spontaneous inflammasome activation by pyrin S242R.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.

ArfGAP1 expression alters GBF1 recruitment to Golgi membranes.

High-affinity mutants of β3 integrin fail to stimulate RhoA activity and fibronectin fibrillogenesis. High-affinity mutants of β3 integrin fail to stimulate.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

Dissociation of HP1α from H3K9me3 is critical to potentiation of steady-state ATM activity. Dissociation of HP1α from H3K9me3 is critical to potentiation.

IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.

Lysine residues in the cytoplasmic region of TfR are involved in the MARCH8-induced downregulation of TfR. Lysine residues in the cytoplasmic region of.

Figure 6 Multiple target epitopes exist in the N-terminal domains of Caspr2 (A) Multidomain deletion constructs of Caspr2 were generated to determine which.

WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates TfR. WT MARCH8, but not the CS mutant, specifically ubiquitinates and downregulates.

Vps36 interacts with Smo in the absence of Hh

Mutant MFN2 proteins have normal half-lives and are properly localized to mitochondria. Mutant MFN2 proteins have normal half-lives and are properly localized.

FOXO3a phosphorylation and expression.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

BME treatment increases granzyme B expression in NK 3.3 cells.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila. GATOR1 is required to maintain baseline levels of TORC1 activity in Drosophila.

Targeting DCLK1 by miRNA-137.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Changes in signal transduction pathway induced by gefitinib.

In Vivo Interaction Between AtPIP2;1 and GRFs

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Effect of bevacizumab on the proliferation of A2780 cells.

Fig. 6 The C9ORF72/SMCR8 complex regulates ULK1.

The interaction of PALB2 with BRCA1 is required for the assembly of PALB2, BRCA2, and RAD51 nuclear foci. The interaction of PALB2 with BRCA1 is required.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

BRCA1 sequence variants classified as pathogenic do not restore HR

KDM4A levels affect the distribution of translation initiation factors

Presentation transcript:

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant constructs. DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant constructs. The blots were probed with anti-GFP antibodies. A representative blot is shown. (B) Quantification of protein expression levels from 3 independent Western blot experiments is shown. Actin levels were used for normalization and error bars indicate SD values. *p < 0.05 by Student t test. (C) HeLa cell lysates from cells transfected with GFP-tagged mutant constructs were treated with 20 mM 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) cross-linking agent and analyzed by Western blot. The monomeric and dimeric forms are indicated. WT = wild type. Ryan S. Dhindsa et al. Neurol Genet 2015;1:e4 © 2015 American Academy of Neurology