Fig. 6. Comparison of Plk4 with Sas-6 localization

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An analysis of Plk4 centriolar dynamics.

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Fig. 6. Comparison of Plk4 with Sas-6 localization Fig. 6. Comparison of Plk4 with Sas-6 localization.(A) U2OS cells were stained with antibodies against Plk4 and either Cep152-C (upper panel) or Cep152-N (lower panel); representative 3D-SIM images are shown. Comparison of Plk4 with Sas-6 localization.(A) U2OS cells were stained with antibodies against Plk4 and either Cep152-C (upper panel) or Cep152-N (lower panel); representative 3D-SIM images are shown. Scale bar 0.5 µm. Schematic illustrates relative localizations. (B) Representative 3D-SIM and wide-field (WF) images of cells stained with antibodies against Sas-6, Cep135, Plk4 and CP110 (far-red channel); schematic illustrates orientation of centrioles. Scale bar 0.5 µm. (C) U2OS cells were stained with antibodies against Sas-6, Cep152 and Plk4 and counterstained with DAPI. Representative 3D-SIM and correlative wide-field (WF) images are shown. Scale bars 1 µm or 5 µm (overview). (D) U2OS cells were stained as in C and wide-field microscopy was used to count cells showing the indicated combinations of Plk4 and Sas-6 staining at each cell cycle stage (n = 100 for each cell cycle stage). Katharina F. Sonnen et al. Biology Open 2012;1:965-976 © 2012. Published by The Company of Biologists Ltd