1. Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media.
Fcp1 CTD phosphatase affects a broad range of transcripts in the +N and -N media. (A) Fcp1 chromosomally tagged with GFP was observed in the quiescent -N medium of WT (left) and fcp1-452 mutant (right). Images stained with GFP (top), DAPI (middle) and merged (bottom) are shown. Scale bars: 1 μm. (B) Chromosomally tagged WT Fcp1-GFP and mutant Fcp1-452-GFP were expressed in the quiescent -N medium and detected by immunoblot using antibodies against GFP. The band is indicated by an arrowhead. (C) Transcriptomic patterns of WT and fcp1-452 mutant in the +N or -N medium at 26 and 37°C. Color panel indicates relative increase (red) and decrease (green) to the median (white) of eight different culture conditions for each transcript. Transcripts of proliferating (+N) cultures were obtained by shifting from 26 to 37°C (26°C in control) for 4 hours in the +N medium. Transcripts of quiescent (-N) cultures were obtained by shifting from 26°C (pre-culture for 24 hours in the -N medium) to 37°C (26°C in control) for 24 hours in the -N medium. (D) The number of gene transcripts for each category and overlapped categories.
Kenichi Sajiki et al. J Cell Sci 2009;122:
© The Company of Biologists Limited 2009